诃子超微粉的工艺优化及其对黄嘌呤氧化酶活性的影响

Optimization of the process of superfine powder of Terminalia chebula Retz. and its effect on the activity of xanthine oxidase

中文关键词:  诃子  超微粉  优化  制备工艺  黄嘌呤氧化酶

英文关键词:Terminalia chebula Retz.  superfine powder  optimization  preparation process  xanthine oxidase

中文摘要:

      目的 优化诃子超微粉的制备工艺, 探究其对黄嘌呤氧化酶(xanthine oxidase, XOD)活性的影响。方法 以XOD抑制率为评价指标, 在单因素实验考察球料比、冷冻时间、转速、球磨时间的基础上, 采用正交实验优化制备工艺, 并对制备的超微粉进行粉体学性质检测, 并评价其XOD抑制率。建立优化后的诃子超微粉的高效液相色谱(high performance liquid chromatography, HPLC)指纹图谱, 并与原粉末进行对比, 并对粉体学性质进行检测。结果 采用球磨法制备了诃子超微粉, 诃子超微粉最优制备条件为: 球料比5:1、冷冻时间40 min、转速400 r/min、球磨时间1.0 h, 优化后的超微粉粒径为22.42 μm, 5 mg/mL XOD抑制率为69.10%。优化后的诃子超微粉可显著改善普通粉的粉体学性质, 降低药粉粒径; 经HPLC分析, 两种诃子粉末指纹图谱主要成分基本一致, 超微粉碎工艺未破坏诃子成分。结论 优化后的诃子超微粉抗XOD活性更佳, 为诃子超微粉在高尿酸血症中的应用提供了基础。

英文摘要:

      Objective To optimize the preparation technology of superfine powder of Terminalia chebula Retz. and explore its effect on xanthine oxidase (XOD) activity. Methods Using XOD inhibition rate as the evaluation index and ball material ratio, freezing time, rotational speed and ball milling time as the influencing factors, orthogonal experiments were conducted to optimize the ultramicro pulverization process of Terminalia chebula Retz. The optimized ultramicro powder was tested for its powder ological properties to optimize its preparation process. The optimized high performance liquid chromatography (HPLC) fingerprint of Terminalia chebula Retz. superfine powder was established, compared with the original powder, and the powder properties were detected. Results The ultrafine powder of Terminalia chebula Retz. was prepared by ball milling. The optimum preparation conditions of the superfine powder were: Ball-to-material ratio of 5:1, freezing time of 40 min, rotational speed of 400 r/min, and ball milling time of 1.0 h. The optimized superfine powder particle size was 22.42 μm, and the XOD inhibition rate was 69.10% at 5 mg/mL. The optimized Terminalia chebula Retz. superfine powder could remarkably improve the powder mechanical properties of common powder and reduce the particle size of the powder; HPLC analysis showed that the main components in the fingerprint chromatograms of the two kinds of Terminalia chebula Retz. powder were basically the same, and the components of Terminalia chebula Retz. were not damaged by the superfine grinding process. Conclusion The optimized superfine powder of Terminalia chebula Retz. has better anti XOD activity, which provides a basis for the application of Terminalia chebula Retz. superfine powder in hyperuricemia.