基于气相色谱-离子迁移谱法分析青霉侵染木耳菌丝的挥发性化合物

Analysis of volatile compounds in mycelia of Auricularia auricula infected by Penicillium sp. based on gas chromatography-ion mobility spectrometry

中文关键词:  黑木耳  桔青霉  歧皱青霉  气相色谱-离子迁移谱法  挥发性化合物

英文关键词:Auricularia auricula  Penicillium citrinum  Penicillium steckii  gas chromatography-ion migration spectrometry  volatile components

中文摘要:

      目的 分析青霉侵染木耳菌丝的挥发性化合物, 发掘潜在的特征标志物。方法 以分离自木耳菌包的桔青霉、歧皱青霉及黑木耳菌丝为研究对象, 采用气相色谱-离子迁移谱法(gas chromatography-ion mobility spectrometry, GC-IMS)分析青霉侵染木耳菌丝不同时期挥发性化合物的种类及变化趋势, 并利用主成分分析(principal component analysis, PCA)比较不同侵染阶段挥发性化合物间的差异。结果 指纹图谱及PCA分析均可以比较明显地区分开青霉侵染木耳菌丝的不同阶段。挥发性化合物主要在桔青霉和歧皱青霉侵染木耳菌丝后的第4~8 d产生, 整体变化趋势基本一致, 共鉴定出41种化合物, 包括酯类、醇类、酮类、醛类及其他类。1-戊醇、乙酸己酯、2,3-戊二酮、2-丁酮、2,3丁二酮、1-丙醇、2-己醇、乙酸乙酯、丁酸乙酯、2-甲基丁酸等挥发性化合物可作为青霉侵染木耳菌丝的潜在标志物, 其中, 1-戊醇、乙酸己酯可作为青霉早期污染木耳菌丝的潜在标志物。3-羟基-2-丁酮可作为桔青霉侵染木耳菌丝后的潜在特征标志物。结论 本研究利用GC-IMS分析获得了两种青霉污染木耳菌丝的潜在特征标志物, 为木耳栽培过程中青霉污染的早期预测提供了一定的理论基础。

英文摘要:

      Objective To analyze the volatile components of mycelia of Auricularia auricula infected by Penicillium sp., and explore the potential characteristic markers. Methods Penicillium citrinum and Penicillium steckii isolated from cultivation bag, and mycelia of Auricularia auricula were used as research objects. The gas chromatography-ion mobility spectrometry (GC-IMS) was adopted to analyze the types and trends of volatile components in fungus mycelia infected by Penicillium sp., and the differences in volatile compounds in different infection stages were further compared by principal component analysis (PCA). Results The fingerprint and PCA analysis could clearly distinguish the different stages of the fungus infection. The volatile components were mainly produced on the 4th to 8th day after the infection of Penicillium citrinum and Penicillium steckii, and overall trend were similar between the 2 Penicillium sp. strains. A total of 41 volatile compounds were identified, mainly included esters, alcohols, ketones, aldehydes, and others, and the volatile components of 1-pentanol, hexyl acetate, 2,3-pentanedione, 2-butanone, 2,3-butanone, 1-propanol, 2-hexanol, ethyl acetate, ethyl butyrate, 2-methyl butyric acid could be regarded as potential markers of mycelia of Auricularia auricula infected by Penicillium sp.. Besides, 1-pentanol and hexyl acetate could be considered as potential markers for early contamination of Auricularia auricular mycelia infected by Penicillium sp.. Additionally, 3-hydroxy-2-butanone could be a potential marker of mycelia infected by Penicillium citrinum. Conclusion The characteristic potential markers of mycelia of Auricularia auricula infected by Penicillium sp. were obtained by GC-IMS, which provides some theoretical foundations for the early prediction of contamination by Penicillium sp. during the cultivation of Auricularia auricula.