基于气相色谱-离子迁移谱法比较刺梨和无籽刺梨挥发性成分差异

Comparison of volatile components of Rosa roxburghii Tratt and Rosa sterilis based on gas chromatography-ion mobility spectrometry

中文关键词:  气相色谱-离子迁移谱法  刺梨  无籽刺梨  挥发性成分

英文关键词:gas chromatography-ion migration spectrometry  Rosa roxburghii Tratt  Rosa sterilis  volatile components

中文摘要:

      目的 比较刺梨和无籽刺梨的挥发性成分差异, 构建两种刺梨挥发性成分的指纹图谱。方法 采用气相色谱-离子迁移谱法(gas chromatography-ion mobility spectrometry, GC-IMS)对刺梨和无籽刺梨的挥发性成分进行分析。通过Gallery Plot软件构建两种刺梨挥发性成分的指纹图谱, 比较两种刺梨挥发性成分的差异, 确定其主要特征挥发性成分。结果 从刺梨和无籽刺梨中共检出73种挥发性成分, 鉴定出56种挥发性成分, 包括酯类25种、醛类15种、酮类8种、醇类4种、萜类2种、酸类1种、硫醚1种。刺梨和无籽刺梨挥发性成分差异显著, 二者差异的主要标志物为香茅醇、壬酸、2-羟基-4-甲基戊酸乙酯、2-甲基丁酸甲酯、2-甲基丁醛、二甲基硫醚。结论 刺梨的挥发性成分比无籽刺梨更为丰富, 二者挥发性成分指纹图谱差异明显, 本研究可为刺梨挥发性成分的研究及刺梨的开发利用提供数据参考。

英文摘要:

      Objective To compare the differences of volatile components of Rosa roxburghii Tratt and Rosa sterilis, and construct the fingerprints of volatile components of 2 kinds of roxburgh rose. Methods The volatile components of Rosa roxburghii Tratt and Rosa sterilis were analyzed by gas chromatography-ion mobility spectrometry (GC-IMS). The fingerprints of volatile components of 2 kinds of roxburgh rose were constructed by Gallery plot software, and the differences of volatile components between the 2 kinds of roxburgh rose were compared to determine the main characteristic volatile components. Results A total of 73 volatile components were detected from Rosa roxburghii Tratt and Rosa sterilis, where a total of 56 volatile components were identified respectively, including 25 kinds of esters, 15 kinds of aldehydes, 8 kinds of ketones, 4 kinds of alcohols, 2 kinds of terpenes, 1 kind of acid and 1 kind of sulfide. The difference in volatile components between the 2 kinds of roxburgh rose were very significant, the differential markers were citronellol, nonanoic acid, ethyl 2-hydroxy-4-methyl pentanoate, methyl 2-methylbutyrate, 2-methylbutana, dimethyl sulfide. Conclusion The volatile components of Rosa roxburghii Tratt are more abundant than Rosa sterilis, and their fingerprints are significantly different, which provides a data reference for the study of volatile components and this work lays a foundation for the development and utilization of Rosa roxburghii Tratt.