祁白芷多糖脱色工艺优化及抗氧化活性研究

Study on optimization of decolorization process and antioxidant activity of polysaccharide from Angelicae dahurica

中文关键词:  祁白芷  多糖  脱色工艺  大孔树脂法  抗氧化活性

英文关键词:Angelica dahurica  polysaccharide  decolorization process  macroporous resin method  antioxidant activity

中文摘要:

      目的 研究祁白芷多糖的脱色工艺及抗氧化活性。方法 采用活性炭脱色法、双氧水脱色法及大孔树脂脱色法3种脱色方法对祁白芷多糖进行脱色, 以脱色率和多糖保留率为考察标准。通过1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)、羟基(hydroxyl radical, ·OH)自由基以及还原能力的测定, 对比祁白芷多糖的脱色前后抗氧化活性。结果 对比3种脱色方法, 选择脱色效果较优的大孔树脂脱色法, 通过正交实验设计得出大孔树脂脱色法的最优条件为: 多糖与大孔树脂湿重的质量比为1:25、脱色温度为20℃、调节pH为6、脱色时间3 h, 在此条件下, 多糖脱色率为56.22%, 多糖保留率为75.34%。且脱色前后的祁白芷多糖均具有一定的抗氧化活性。结论 大孔树脂脱色法对祁白芷多糖的脱色效果较于其他两种的更优, 且脱色前的祁白芷多糖抗氧化活性优于脱色后的。为改善祁白芷多糖的外观和将其开发为抗氧化剂提供了前期基础。

英文摘要:

      Objective To investigate the decolorization process and antioxidant activity of Angelicae dahurica polysaccharides. Methods Three decolorization methods including activated carbon, hydrogen peroxide and macroporous resin were used to decolorize the polysaccharides of Angelica dahurica. The criteria were based on the rate of decolorization and the retention rate of polysaccharide. 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical (·OH) and reducing ability were determined to compare the antioxidant activities of Angelica dahurica polysaccharides before and after decolorization. Results The three decolorization methods were compared, and the macroporous resin decolorization method with better decolorization effect was selected. Through orthogonal experimental design, the optimal conditions of the macroporous resin decolorization method were obtained as follows: The ratio of polysaccharide mass to macroporous resin wet weight was 1:25, decolorization temperature was 20℃, the pH was adjusted to 6 and decolorization time was 3 h. Under this condition, the decolorization rate of polysaccharide was 56.22% and the retention rate of polysaccharide was 75.34%. The polysaccharides of Angelica dahurica before and after decolorization had antioxidant activity. Conclusion The decoloration effect of the macroporous resin decoloration method on Angelicae dahuricae polysaccharide is more significant than the other two, and the antioxidant activity of Angelicae dahuricae polysaccharide before decoloration is better than that after decoloration. In order to improve the appearance of Angelicae dahuricae polysaccharide and study it as an antioxidant, it provides a foundation.