9个品种番石榴果实提取物抗氧化活性比较研究

Antioxidant activities of fruit extracts from 9 kinds of cultivars of Psidium guajava L.

中文关键词:  番石榴  总酚  黄酮  抗氧化活性  相关性分析

英文关键词:Psidium guajava L.  total phenols  flavonoids  antioxidant activity  correlation analysis

中文摘要:

      目的 分析比较9个品种番石榴果实的抗氧化活性, 为筛选适合开发天然抗氧化产品的番石榴优良品种提供理论依据。方法 测定‘紫色’‘红宝石’‘粉红蜜’‘西瓜’‘珍珠’‘帝王’‘翠玉’‘水蜜’‘本土’9个品种番石榴醇提物和水提物的总酚、黄酮含量, 研究其对2,2’-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐[2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonicacid) ammonium salt, ABTS]、1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2- picrylhydrazyl, DPPH)自由基清除能力和总抗氧化能力, 利用抗氧化能力综合指数评价不同样品的抗氧化活性, 并分析总酚、黄酮含量与抗氧化活性的相关性。结果 9个品种番石榴醇提物总酚和黄酮含量分别为1.363~2.394 g/100 g和0.607~1.875 g/100 g, 对DPPH、ABTS自由基的半数清除浓度(medium inhibition concentration, IC50)分别为0.242~0.518 mg/mL和1.990~4.929 mg/mL, 总抗氧化能力为0.113~0.206 mmol/g; 水提物总酚和黄酮含量则为1.192~2.142 g/100 g和0.393~1.118 g/100 g, 对DPPH、ABTS自由基的IC50分别为0.320~0.661 mg/mL和2.837~5.472 mg/mL, 总抗氧化能力为0.105~0.181 mmol/g。水提物和醇提物的总酚、黄酮含量均以‘紫色’番石榴最高, 抗氧化能力综合指数也以‘紫色’最高。除‘水蜜’外, 其他品种醇提物的抗氧化活性均高于水提物。相关性分析结果显示, 总酚、黄酮含量与DPPH自由基清除能力、ABTS自由基清除能力、总抗氧化能力和抗氧化能力综合指数呈正相关关系。结论 9个品种番石榴的提取物中, ‘紫色’醇提物的总酚、黄酮含量和抗氧化活性最高, 是开发天然抗氧化产品的最佳来源。

英文摘要:

      Objective To analyze and compare the antioxidant activities of 9 kinds of cultivars of Psidium guajava L. fruit, and to provide a theoretical basis for screening excellent varieties of Psidium guajava L. suitable for developing natural antioxidant products. Methods The content of total phenols and flavonoids of ethanol extracts and aqueous extracts from 9 kinds of cultivars of Psidium guajava L. (‘Zise’ ‘Hongbaoshi’ ‘Fenhongmi’ ‘Xigua’ ‘Zhenzhu’ ‘Diwang’ ‘Cuiyu’ ‘Shuimi’ ‘Bentu’) was determined, and the scavenging abilities towards 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonicacid) ammonium salt (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical, total antioxidant capacity were studied, furthermore, an overall antioxidant potency composite index was calculated to evaluate the antioxidant activity, then the correlations between the content of phenols and flavonoids with antioxidant activities were analyzed. Results The content of total phenols and flavonoids of ethanol extracts from 9 kinds of cultivars of Psidium guajava L. was 1.363?2.394 g/100 g and 0.607?1.875 g/100 g respectively, and the medium inhibition concentration (IC50) towards DPPH and ABTS free radicals were 0.242?0.518 mg/mL and 1.990?4.929 mg/mL, then the total antioxidant capacities were 0.113?0.206 mmol/g. The content of total phenols and flavonoids of aqueous extracts from 9 kinds of cultivars of Psidium guajava L. was 1.192?2.142 g/100 g and 0.393?1.118 g/100 g respectively, and the IC50 towards DPPH and ABTS free radicals was 0.320?0.661 mg/mL and 2.837?5.472 mg/mL, then the total antioxidant capacities were 0.105?0.181 mmol/g. Both ethanol and aqueous extracts from ‘Zise’ Psidium guajava L. had the highest content of total phenols and flavonoids as well as antioxidant potency composite index. The antioxidant activities of ethanol extracts were generally higher than aqueous extracts for different cultivars except ‘Shuimi’. The correlation analysis indicated that the content of total phenols and flavonoids was positively correlated with DPPH free radical scavenging ability, ABTS free radical scavenging ability, total antioxidant capacity and antioxidant potency composite index. Conclusion Among the extracts from 9 kinds of cultivars of Psidium guajava L., ethanol extract of ‘Zise’ has the highest content of total phenols and flavonoids as well as the strongest antioxidant activity, therefore, it is possible to be the optimum source to develop natural antioxidant products.