马关县不同产地草果氨基酸组成与分析

Amino acid composition and analysis of Amomum tsaoko from different producing areas in Maguan County

中文关键词:  草果  氨基酸  比较分析

英文关键词:Amomum tsaoko  amino acids  comparison and analysis

中文摘要:

      目的 研究马关县不同产地草果中氨基酸含量与组成。方法 样品经酸水解法处理后, 使用Biochrom 30+型全自动氨基酸分析仪测定其含量, 分析5个乡镇产草果中的总氨基酸、必需氨基酸评分、氨基酸比值系数和比值系数分, 与世界卫生组织和联合国粮农组织建议的氨基酸评分标准模式进行对比, 评价草果果实的营养价值。结果 草果M2、M3、M5检出17种氨基酸和7种必需氨基酸; 草果M1、M4检出16种氨基酸, 未检出蛋氨酸。草果总氨基酸含量为36.02~45.79 mg/g, 必需氨基酸含量为13.21~16.42 mg/g, 以谷氨酸(6.67 mg/g)、天冬氨酸(4.72 mg/g)含量较高。氨基酸比值系数法显示草果中蛋氨酸+胱氨酸、赖氨酸均为不足, 亮氨酸、苯丙氨酸+酪氨酸、缬氨酸均为过剩, 第一限制性氨基酸为蛋氨酸+胱氨酸。草果中呈味类氨基酸和药用氨基酸含量丰富, 分别占总氨基酸的89%、64%。氨基酸比值系数分显示草果M2得分最高, 为65.40。结论 草果氨基酸含量丰富且以谷氨酸、天冬氨酸含量较高。综合分析以马关县八寨镇田中村种植的草果蛋白质中氨基酸营养价值较高。

英文摘要:

      Objective To study the content and composition of amino acids in Amomum tsaoko from different areas in Maguan County. Methods After the samples were treated by acid hydrolysis method, the content was determined by Biochrom 30+ full-automatic amino acid analyzer, and the total amino acids, essential amino acid scores, amino acid ratio coefficients and ratio coefficient scores of Amomum tsaoko produced in 5 townships were analyzed. The nutritional value of Amomum tsaoko was evaluated by comparing them with the amino acid scoring standard mode recommended by the World Health Organization and the Food and Agriculture Organization of the United Nations. Results The 17 kinds of amino acid and 7 kinds of essential amino acid were detected in M2, M3 and M5, while 16 kinds of amino acid in M1 and M4, with no methionine were found. The content of total amino acids was ranged from 36.02 to 45.79 mg/g, with essential amino acids from 13.21 to 16.42 mg/g, among which the glutamic acid (6.67 mg/g) and aspartic acid (4.72 mg/g) were the higher. The amino acid ratio coefficients showed that methionine+cystine, lysine were insufficient, while leucine, phenylalanine+tyrosine, valine were excessive, the first limiting amino acid was methionine+cystine. The content of flavorful amino acids and medicinal amino acids was abundant, whose proportion were 89% and 64% of total amino acids, respectively. The amino acid ratio coefficient scores showed that M2 was the highest (65.40) among all groups. Conclusion The Amomum tsaoko is rich in amino acids with glutamic acid and aspartic acid much higher. According to the comprehensive analysis, growing in Tianzhong Village, Bazhai Town, Maguan County has the higher amino acid nutritional value.