基于体外模拟消化模型评价刺参中3种拟除虫菊酯的生物可给性

Evaluation of bioavailabilities of 3 kinds of pyrethroids in Stichopus japonicas based on in vitro simulated digestion models

中文关键词:  拟除虫菊酯  生物可给性  刺参  体外模拟消化

英文关键词:pyrethroid  bioavailability  Stichopus japonicas  in vitro simulated digestion

中文摘要:

      目的 利用体外模拟消化模型评估刺参(Stichopus japonicas)中3种拟除虫菊酯的生物可给性。方法 基于胃相动态pH和静态肠相的体外模拟消化模型, 采用气相色谱法分析加工前后刺参中3种拟除虫菊酯(甲氰菊酯、氯氰菊酯和溴氰菊酯)的含量。结果 加工组与原料组相比, 经过胃相消化后, 3种菊酯类含量均显著降低(P<0.05); 无论原料组还是加工组, 甲氰菊酯和溴氰菊酯的浓度在肠道模拟消化后均显著低于消化前(P<0.05); 原料组中甲氰菊酯、氯氰菊酯和溴氰菊酯的生物可给性分别为63.09%、86.67%和71.83%, 除了溴氰菊酯, 加工后另外两种菊酯类生物可给性均低于原料组。结论 刺参中3种拟除虫菊酯经模拟消化后均不能从食物基质中完全释放, 此外加热处理可以在一定程度上降低刺参中拟除虫菊酯的生物可给性。

英文摘要:

      Objective To evaluate the bioavailabilities of 3 kinds of pyrethroids in Stichopus japonicas by an in vitro simulated digestion model. Methods Based on an in vitro simulated digestion model with dynamic pH in the gastric phase and static intestinal phase, the content of 3 kinds of pyrethroids (fenpropathrin, cypermethrin and deltamethrin) in Stichopus japonicas was analyzed by gas chromatography before and after processing. Results Compared with the raw material group, the content of 3 kinds of pyrethroids in the processed group was all significantly decreased after gastric digestion (P<0.05). No matter in the raw material group or the processing group, the concentrations of fenpropathrin and deltamethrin were significantly decreased after intestinal simulated digestion compared with those before digestion (P<0.05). The bioavailabilities of fenpropathrin, cypermethrin and deltamethrin in the raw materials group were 63.09%, 86.67% and 71.83%, respectively. Except deltamethrin, the bioavailabilities of the other 2 kinds of pyrethroids after processing were lower than that of the raw material group. Conclusion None of the 3 kinds of pyrethroids in Stichopus japonicas can be completely released from the food matrix after simulated digestion, and in addition, heating treatment can reduce the bioavailabilities of pyrethroids in Stichopus japonicas to some extent.