60Co-γ射线辐照技术优化小龙虾虾壳酶解工艺

Optimization of enzymatic hydrolysis process of Procambarus clarkii shells by 60Co-γ ray irradiation technology

中文关键词:  小龙虾  60Co-γ射线  改性蛋白酶  酶解液  工艺优化

英文关键词:Procambarus clarkii  60Co-γ ray  modified protease  enzymatic solution  process optimization

中文摘要:

      目的 通过辐照技术优化小龙虾虾壳酶解工艺。方法 采用60Co-γ射线辐照碱性蛋白酶使其改性, 通过调节加酶量、pH、酶解温度、酶解时间和酶解液辐照剂量, 比较水解度和蛋白质提取率, 得到最佳酶解条件, 进而利用水解度响应面实验优化酶解的工艺参数。结果 在加酶量为5200 U/g、pH 8.5、酶解温度为60.5℃、酶解时间为180 min、酶解液辐照剂量为1 kGy时酶解效果最佳。其中, 最佳酶解效果的水解度为28.94%, 蛋白质提取率为85.13%。结论 60Co-γ射线辐照技术改性碱性蛋白酶能有效提高小龙虾虾壳的酶解效果。

英文摘要:

      Objective To optimize the enzymatic hydrolysis process of Procambarus clarkii shells by 60Co-γ ray irradiation technology. Methods Alkaline protease was modified by 60Co-γ ray irradiation. The degrees of hydrolysis and protein extraction rates were compared by adjusting the amount of enzyme added, pH, enzymolysis temperature, enzymolysis time and irradiation dose of enzymolysis solution, so as to obtain the best enzymolysis conditions. Then, the hydrolysis degree response surface experiment was used to optimize the process parameters of enzymolysis. Results The optimum conditions were as follows: Enzyme dosage of 5200 U/g, pH of 8.5, enzymolysis temperature of 60.5℃, enzymolysis time of 180 min and irradiation dose of enzymolysis solution of 1 kGy. Among them, the hydrolysis degree of the best enzymolysis effect was 28.94%, and the extraction rate of protein was 85.13%. Conclusion The modification of alkaline protease by 60Co-γ ray irradiation technology can effectively improve the enzymolysis effect of Procambarus clarkii shells.