水产品中人鱼共患细菌性病原分子生物学检测方法研究进展

Research progress on molecular biological detection methods for bacterial pathogen common to humans and fish in aquatic products

中文关键词:  水产品  人鱼共患细菌性病原  分子生物学  成簇的规律间隔的短回文重复序列

英文关键词:aquatic products  bacterial pathogen common to humans and fish  molecular biology  clustered regularly interspaced short palindromic repeats

中文摘要:

      水产行业是国民经济重要产业之一, 水产养殖过程中病原微生物造成鱼类病害频发, 人鱼共患病原菌会对人类健康构成威胁, 水产品安全问题日益突出。人鱼共患病原菌检测方法对于病害的发现、预防及水产行业的健康可持续发展具有重要意义。人鱼共患病原菌包括人鱼共患细菌性病原和人鱼共患寄生虫病原等。本文对水产品中常见的人鱼共患细菌性病原的种类及其危害性、细菌性病原分子生物学检测方法的原理和应用及成簇的规律间隔的短回文重复序列(clustered regularly interspaced short palindromic repeats, CRISPR)基因编辑技术在病原菌检测方面的最新研究进展进行了综述, 为研发易携带且简便快速的新型核酸检测方法及产品及水产品中人鱼共患病原菌的检测和防治提供参考和借鉴。

英文摘要:

      The aquatic industry is one of the important industries in the national economy. In the process of aquaculture, pathogenic microorganisms cause frequent fish diseases, and the bacterial pathogen common to humans and fish also pose a threat to human health. The safety of aquatic products is becoming increasingly prominent. The detection methods of bacterial pathogen common to humans and fish is of great significance for the discovery and prevention of diseases and the healthy and sustainable development of the aquatic industry. The pathogenic bacteria common to humans and fish include symbiotic bacterial pathogens and symbiotic parasitic pathogens. This paper reviewed the species and harmfulness of common fish and human bacterial diseases in aquatic products, the principle and application of molecular biology detection methods for bacterial pathogen, and the latest research progress of clustered regularly interspaced short palindromic repeats (CRISPR) gene editing technology in pathogenic bacteria detection, so as to provide references for the research and development of portable, simple and rapid new nucleic acid detection methods, products and the detection and prevention of bacterial pathogen common to humans and fish in aquatic products.