α-乳白蛋白对秀丽隐杆线虫寿命、繁殖力与活力的影响

Effects of α-lactalbumin on the lifespan, fecundity and vitality of Caenorhabditis elegans

中文关键词:  α-乳白蛋白  秀丽隐杆线虫  繁殖力  活力

英文关键词:α-lactalbumin  Caenorhabditis elegans  fecundity  vitality

中文摘要:

      目的 研究α-乳白蛋白对秀丽隐杆线虫寿命、活力和繁殖力的影响。方法 使用不同浓度的α-乳白蛋白(提取自纯牛奶)喂养秀丽隐杆线虫, 并观察其寿命、繁殖力与活力变化。结果 α-乳白蛋白添加量为45 mg/L时, 与对照组相比, 线虫寿命延长到(26.33±0.72) d(增幅59.58%, P<0.05); 平均运动速率达到(149.00±7.79)次/min (增幅19.20%, P<0.05); 平均弯曲度达到了(17.00±1.63)次/20 s(增幅54.54%, P<0.05), 继续增加α-乳白蛋白含量时, 以上指标无显著性变化(P>0.05)。当α-乳白蛋白添加量为30 mg/L时, 平均产卵数相比对照组增加到(236.33±12.23)个(增幅53.13%, P<0.05), 而继续增加α-乳白蛋白含量, 平均产卵数无显著性变化(P>0.05)。结论 α-乳白蛋白喂养对线虫寿命延长、繁殖能力提升、运动能力保持(减缓衰退)等具有一定的作用。

英文摘要:

      Objective To study the effects of α-lactalbumin on lifespan, viability and fecundity of Caenorhabditis elegans. Methods Caenorhabditis elegans were fed with α-lactalbumin (extracted from pure milk) at different concentrations, and the changes of lifespan, fecundity and vitality were observed. Results When the supplemental level of α-lactalbumin was 45 mg/L, compared with the control group, the lifespan of nematodes was extended to (26.33±0.72) d (increased by 59.58%, P<0.05). The average exercise rate was (149.00±7.79) times/min (increased by 19.20%, P<0.05). The average bending degree reached (17.00±1.63) times/20 s (increased by 54.54%, P<0.05). When continuing to increase the content of α-lactalbumin, there was no significant change in the above indicators (P>0.05). When the α-lactalbumin supplemental level was 30 mg/L, compared with the control group, the average spawning number increased to (236.33±12.23) (increased by 53.13%, P<0.05), However, there was no significant difference in average oviposition number when α-lactalbumin content was continuously increased (P>0.05). Conclusion α-lactalbumin feeding was proved to have a certain role in prolonging the life of nematodes, improving reproduction capabilities, and maintenance athletic ability (slowing down the decline caused by aging included).