食品和临床来源金黄色葡萄球菌对Caco-2细胞侵袭力的比较研究

Comparative study the invasiveness on Caco-2 cells of Staphylococcus aureus strains isolated from food and clinical samples

中文关键词:  金黄色葡萄球菌  不同克隆系  Caco-2细胞  细胞侵袭力  菌膜形成能力

英文关键词:Staphylococcus aureus  different clonal complex  Caco-2 cells  invasiveness capability  biofilm formation ability

中文摘要:

      目的 研究食品和临床来源金黄色葡萄球菌不同克隆系(clonal complex, CC)菌株对Caco-2细胞的侵袭力和菌膜形成能力。方法 采用Caco-2细胞体外培养方法、庆大霉素与溶葡萄球菌酶保护试验, 研究金黄色葡萄球菌12个不同来源的克隆系菌株对人体肠道表皮细胞的体外侵袭力; 采用96孔板结晶紫染色法评估不同菌株的菌膜形成能力。结果 体外侵袭能力试验结果表明CC5、CC25、CC30、CC50、CC59、CC239和CC398等克隆系都具有细胞强侵袭株, 与牲畜特异相关的CC9也展现出细胞强侵袭力, 而CC1、CC20、CC72和CC121等克隆系则明显具有较弱的细胞侵袭能力。不同克隆系菌株菌膜形成的能力也具有一定差异, 其中CC5、CC9、CC25和CC239等克隆系菌膜形成能力相比较强, 而CC1、CC72、CC59和CC121等则菌膜形成能力较弱。进一步分析发现不同克隆系菌株的菌膜形成能力与细胞侵袭力呈现正相关(R=0.743)。结论 金黄色葡萄球菌不同克隆系菌株的细胞侵袭能力、菌膜形成能力具有一定差异, 为有效防控金黄色葡萄球菌临床感染提供重要支持。

英文摘要:

      Objective To investigate the invasiveness on Caco-2 cells and biofilm formation ability of different clonal complex (CC) of Staphylococcus aureus strains isolated from food and clinical samples. Methods Caco-2 cells were cultured in vitro, and gentamicin combined with lysostaphin protection test were used to explore the invasiveness ability in vitro of clonal strains of Staphylococcus aureus from 12 different sources on human intestinal epidermal cells; 96-well plate crystal violet staining method was used to study the biofilm-forming ability of different strains. Results The results of in vitro invasion capabilities indicated that strong Caco-2 cell invasiveness strains were found in CC5、CC25、CC30、CC50、CC59、CC239 and CC398. In addition, some isolates belonging to livestock-associated lineage CC9 also showed strong invasion ability. In contrast, there was no strong invasiveness strains in CC1, CC20, CC72 and CC121. The biofilm-forming ability of different clonal lineages had obvious differences, among which the CC5, CC9, CC25 and CC239 showed stronger biofilm forming ability, while the strains of CC1, CC59, CC72 and CC121 had a less capable of biofilm forming ability. In addition, the biofilm formation ability and Caco-2 cell invasiveness ability of Staphylococcus aureus strains were positively associated (R=0.743). Conclusion The cell invasiveness ability and biofilm formation ability of Staphylococcus aureus belonging to different clone lineage has a certain differences, which can provide an important support for the effective prevention and control of clinical infection.