动物源性食品中甲基睾酮残留检测方法研究进展

Research progress on the detection methods of methyltestosterone residues in animal-derived foods

中文关键词:  动物源性食品  甲基睾酮  残留检测

英文关键词:animal-derived food  methyltestosterone  residues detection

中文摘要:

      甲基睾酮是一种激素类药物, 在畜牧业和水产养殖业中作为动物促生长剂和性别逆转剂被使用。我国农业农村部235号公告规定了在所有动物组织中不得检出甲基睾酮, 但是目前还存在违禁使用的情况, 因此有必要对其残留量进行检测。本文主要概述了甲基睾酮的作用机制、危害和法规标准, 系统综述了现有畜禽、水产品中甲基睾酮残留检测常用的样品前处理方法与分析方法。样品前处理方法的确定包括提取试剂的选择、净化方法的优化等, 检测方法主要包括高效液相色谱法、液相色谱-串联质谱法和免疫分析方法等。本文分析了不同检测方法的优缺点、适用范围以及选择依据, 并对检测方法进行了展望, 旨在为加强动物源性食品中甲基睾酮的残留监测提供参考。

英文摘要:

      Methyltestosterone is a kind of hormone drug, which is used as growth promoters and sex reversal agents in the aquaculture and livestock industry. Announcement No.235 of the Ministry of Agriculture and Rural Affairs of China stipulates that methyltestosterone should not be detected in all animal tissues, but it is still prohibited to use it at present, so it is necessary to detect its residues. This paper mainly summarized the action mechanism, harm, regulatory standards of methyltestosterone, systematically described the existing sample pretreatment methods and analysis methods of methyltestosterone residue detection in livestock and aquatic species. Determination of sample pretreatment methods include the selection of extraction reagents, optimization of purification methods and etc., the detection methods of methyltestosterone residues in animal-derived foods mainly include high performance liquid chromatography, liquid chromatography-tandem mass spectrometry and immunoassay etc. Meanwhile, this paper analyzed the application scope, advantages and disadvantages and selection basis of different detection methods, and looked forward to the detection methods, so as to provide reference for strengthening the monitoring of methyltestosterone residues in animal-derived foods.