海藻酸钠-微晶纤维素在SETR中包埋纳豆芽孢杆菌的固定化研究

Immobilization study on the encapsulation of Bacillus subtilis subsp. Natto in SETR by sodium alginate-microcrystalline cellulose

中文关键词:  海藻酸钠  微晶纤维素  新型软弹式反应器  纳豆芽孢杆菌  包埋  pH

英文关键词:sodium alginate  microcrystalline cellulose  soft elastic tubular reactor  Bacillus subtilis subsp. Natto  encapsulation  pH

中文摘要:

      目的 考察海藻酸钠(sodium alginate, SA)-微晶纤维素(microcrystalline cellulose, MC)在新型软弹式反应器(soft elastic tubular reactor, SETR)中对纳豆芽孢杆菌的包埋作用。方法 使用SA与MC制成海藻酸钠-微晶纤维素水凝胶(SA-MC)并包埋纳豆芽孢杆菌, 以光学显微镜形态、微球直径为指标, 单因素法优化SA与MC配比和包埋时间; 并对优化好的SA-MC进行pH溶胀实验; 并观察SA-MC在SETR发酵过程中对pH的反应。结果 SA与MC以1:3 (V:V)的比例混合, 包埋时间1 h效果最佳; SA-MC对pH不敏感, 可用于发酵环境对菌株的包埋和保护; 与空白相比, SA-MC在SETR发酵环境中对pH有缓冲效果。结论 在SETR环境中SA-MC对纳豆芽孢杆菌表现出较好的包埋作用。本研究为天然材料包埋益生菌及SETR的应用推广提供理论支持。

英文摘要:

      Objective To investigate the effect of sodium alginate (SA)-microcrystalline cellulose (MC) on the encapsulation of Bacillus subtilis subsp. Natto in a novel soft elastic tubular reactor (SETR). Methods SA and MC were used to make a hydrogel (SA-MC) encapsulating with Bacillus subtilis subsp. Natto, and the ratio of SA to MC and the encapsulation time were optimized by a single-factor method with light microscope morphology and microsphere diameter as indicators. The pH swelling experiment of optimized SA-MC was carried out, the reaction of SA-MC to pH during SETR fermentation was also observed. Results When SA and MC were mixed in the ratio of 1:3 (V:V) and immobilized for 1 h, encapsulating effect was best. SA-MC was not sensitive to pH and could be used for encapsulation and protection of strains in the fermentation environment; SA-MC had a buffering effect on pH in the SETR fermentation environment compared to the control group. Conclusion SA-MC shows good performance in encapsulating Bacillus subtilis subsp. Natto in SETR environment. This study can provide theoretical supports for the study of probiotics embedded in natural materials and application and popularization of SETR.