微波辅助孵育法富集麦胚多肽的工艺优化

Optimization of incubation parameters for microwave-assisted wheat germ polypeptide enrichment

中文关键词:  胚芽  微波辅助  内源酶活化  多肽含量

英文关键词:germ  microwave-assisted  endogenous enzyme activation  polypeptide content

中文摘要:

      目的 采用微波处理与孵育法结合探索影响麦胚多肽微波孵育的工艺条件。方法 以麦胚为实验原料, 采用微波辅助预处理方法, 研究孵育温度、时间、pH及料液比4种因素对孵育后的蛋白酶活力及多肽含量的影响, 通过单因素和响应面实验进行工艺条件优化。结果 与未进行微波辅助预处理的样品相比, 微波辅助处理(600 W, 10 s)能显著提高(P<0.05)孵育后样品中的蛋白酶酶活力(约9.4倍)和多肽含量(约3.1倍)。经过微波辅助处理后, 孵育温度为51.5℃、pH为4.0、时间为6.33 h、料液比为1:7 (g:mL)时, 蛋白酶活力达最高为3826.23 U/g; 孵育温度为45.0℃、pH为4.8、时间为8.00 h、料液比为1:7 (g:mL)时, 多肽含量达最高为261.23 mg/g。结论 微波辅助处理能有效地激活麦胚孵育液中的内源性蛋白酶活性, 促进蛋白水解反应, 显著提高孵育后的多肽含量。

英文摘要:

      Objective To explore the conditions affecting the microwaveassisted wheat germ polypeptide enrichment. Methods Fresh wheat germ was used as raw material, and microwave-assisted pretreatment was used to study the effects of incubation temperature, time, pH and material liquid ratio on protease activity and polypeptide content after incubation. The process conditions were optimized by single factor and response surface test. Results Compared with the samples without microwave-assisted pretreatment, microwave-assisted treatment (600 W, 10 s) could significantly improve (P<0.05) the protease activity about 9.4 times and polypeptide content about 3.1 times of incubated samples. After microwave-assisted treatment, the maximum protease activity was 3826.23 U/g under the temperature of 51.5℃, pH of 4.0, time of 6.33 h and the ratio of material to liquid (g:mL) of 1:7. And the polypeptide content reached the highest of 261.23 mg/g, when the incubation temperature was 45.0℃, pH was 4.8, time was 8.00 h and the ratio of material to liquid (g:mL) was 1:7. Conclusion Microwave-assisted treatment can significantly activate the endogenous protease activity in wheat incubation solution, promote the level of protein hydrolysis reaction, and significantly improve the polypeptide content during incubation.