沙丁胺醇分子印迹电化学传感器的制备及应用

Fabrication and application of salbutamol molecularly imprinted electrochemical sensor

中文关键词:  沙丁胺醇  纳米金粒子  分子印迹技术  电化学传感器

英文关键词:salbutamol  gold nanoparticles  molecular imprinting technology  electrochemical sensor

中文摘要:

      目的 构建沙丁胺醇(salbutamol, Sal)分子印迹电化学传感器, 并应用于猪肉中Sal的快速测定。方法 选用电聚合法制备Sal的分子印迹膜。首先通过恒电位沉积法(?0.2 V)将纳米金粒子(gold nanoparticles, AuNPs)原位沉积到玻碳电极(glassy carbon electrode, GCE)表面, 制得AuNPs/GCE, 利用循环伏安法(cyclic voltammetry, CV)将功能单体邻苯二胺与Sal聚合在AuNPs/GCE表面, 制备分子印迹聚合物膜。使用0.5 mol/L H2SO4洗脱印迹膜, 得到特异性识别Sal的Sal分子印迹传感器(Sal-MIP/AuNPs/GCE)。采用CV、交流阻抗法(electrochemical impedance spectroscopy, EIS)和差分脉冲伏安法(differential pulse voltammetry, DPV)对传感器的性能进行考察。结果 该传感器在1.0×10?11~1.0×10?6 mol/L浓度范围内对Sal具有较好的响应, 线性方程为ΔIP (μA)=34.079+2.9048lgc (mol/L), 相关系数(r2)为0.9911, 检出限为1×10?11 mol/L, 加标回收率为94.40%~107.00%, 相对标准偏差(relative standard deviations, RSDs)为2.1%~3.5%。结论 该方法具有检测成本低、操作简单、灵敏度高, 能够应用于猪肉中Sal准确快速测定, 有较好的应用价值。

英文摘要:

      Objective To fabricate the salbutamol (Sal) molecular imprinted electrochemical sensor for the rapid determination of Sal in the pork. Methods The molecular imprinted film of Sal was prepared by electrochemical polymerization. Firstly, AuNPs/GCE was prepared by in-situ deposition of gold nanoparticles (AuNPs) on the surface of glassy carbon electrode (GCE) by potentiostatic deposition at the potential of ?0.2 V, then, the functional monomer O-phenylenediamine and Sal were electropolymerized on the surface of AuNPs/GCE by cyclic voltammetry (CV) to prepare molecularly imprinted polymer membrane. After elution with 0.5 mol/L H2SO4, the molecularly imprinted electrochemical sensor (Sal-MIP/AuNPs/GCE) for specific recognition of Sal was obtained. CV, electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were used to investigate the performance of the sensor. Results The peak current was linearly related to the concentration of Sal in the range of 1.0×10?11~1.0×10?6 mol/L with the linear equation of ΔIP (μA)=34.079+2.9048lgc (mol/L), the correlation coefficient (r2) was 0.9911, the proposed sensor could selectively and sensitively detect Sal with the limit of detection of 1×10?11 mol/L, the recoveries were from 94.40% to 107.00%, the relative standard deviations (RSDs) were ranged from 2.1% to 3.5%. Conclusion This method has the advantages of low cost, simple operation and high sensitivity, and can be applied to the accurate and rapid determination of Sal in pork and has good application value.