胶体金免疫层析法快速检测果蔬中的三唑酮及其代谢物残留

Rapid determination of triadimefon and its metabolite residues in fruits and vegetables by colloidal gold immunochromatographic assay

中文关键词:  三唑酮  胶体金  免疫层析法  果蔬

英文关键词:triadimefon  colloidal gold  immunochromatography  fruits and vegetables

中文摘要:

      目的 建立胶体金免疫层析法快速检测蔬菜水果中三唑酮及其代谢物农药残留的分析方法。方法 采用胶体金标记三唑酮单克隆抗体, 利用胶体金的可视化及抗体与抗原的特异性结合, 实现对果蔬中三唑酮农药残留的定性和半定量检测。结果 本方法在层析液中三唑酮及其代谢物的总残留量检出限为2 ng/mL, 对黄瓜和香蕉中三唑酮及其代谢物的总残留量检出限分别为0.05和0.85 mg/kg, 均低于国家标准食品中三唑酮农药最大残留限量(0.1~1 mg/kg), 该试纸条对其他农药无交叉反应, 重复性良好。结论 该方法具有高灵敏度、高特异性, 可快速准确地检测大批量样品中的三唑酮及其代谢物农药残留, 能够满足应急超标事件的现场检测需求。

英文摘要:

      Objective To establish a method for the rapid determination of triadimefon and its metabolite pesticide residues in fruits and vegetables by colloidal gold immunochromatographic assay. Methods Using colloidal gold labeled monoclonal antibody of triadimefon, and then using colloidal gold visualization and specific binding of antibody to antigen, the qualitative and semi-quantitative detection of triadimefon pesticide residues in fruits and vegetables was realized. Results The limits of detection of triadimefon and its metabolite residues in chromatographic liquid were 2 ng/mL, 0.05 and 0.85 mg/kg in cucumber and banana, respectively, which were lower than the maximum residue limit of triadimefon residue in fruits and vegetables specified in the national standard (0.1?1 mg/kg). The test strip had no cross-reaction with other pesticides and had good repeatability. Conclusion This method shows high sensitivity, high specificity, and can quickly and accurately detect triadimefon and its metabolite pesticide residues in a large number of samples, which can meet the needs of on-site detection of emergency events exceeding the maximum residue limit.