新型基质分散挤压萃取-高效液相色谱法测定辣椒粉中6种苏丹红色素

Determination of 6 kinds of Sudan dyes in chilli powder by novel matrix dispersion squeezing extraction-high performance liquid chromatography

中文关键词:  苏丹红色素  基质分散挤压萃取  高效液相色谱法  辣椒粉

英文关键词:Sudan dyes  matrix dispersion squeezing extraction  high performance liquid chromatography  chilli powder

中文摘要:

      目的 建立新型基质分散挤压萃取-高效液相色谱法(matrix dispersion squeezing extraction-high performance liquid chromatography, MDSE-HPLC)测定6种辣椒粉中苏丹红色素的方法。方法 样品与分散剂混匀后, 加入提取剂充分研磨, 通过挤压收集提取溶剂, 经Al2O3固相萃取柱净化, 采用高效液相色谱法进行检测, 外标法定量。结果 6种苏丹红色素在1.2~9.6 μg/g浓度范围内具有良好的线性关系, 相关系数均大于0.998, 检出限为0.10~0.15 μg/g, 定量限为0.30~0.45 μg/g。样品在3个水平加标浓度(1.2、4.8、9.6 μg/g)下的平均回收率为72.0%~102.4%, 相对标准偏差为1.9%~6.8%。结论 本方法简单新颖、成本低、污染少, 能快速有效提取目标物, 准确度和精密度符合要求, 可用于辣椒粉中的6种苏丹红色素的检测。

英文摘要:

      Objective To establish a method for determination of 6 kinds of Sudan dyes in chilli powder by novel matrix dispersion squeezing extraction-high performance liquid chromatography (MDSE-HPLC). Methods After the sample was mixed with dispersant, the extraction reagent was added and fully grinded. The extracting solvent was collected by extrusion, purified by Al2O3 solid phase extraction column, then analyzed by high performance liquid chromatography. The external standard method was used for quantification. Results The calibration curves of 6 kinds of Sudan pigments showed good linearity in the concentration range of 1.2?9.6 μg/g, and the correlation coefficients were greater than 0.998. The limits of detection and quantification were 0.10?0.15 μg/g and 0.30?0.45 μg/g. The average recoveries of the samples at three spiked concentrations (1.2, 4.8, 9.6 μg/g) were 72.0%?102.4%, and the relative standard deviations were 1.9%?6.8%. Conclusion This method is simple, novel, low cost and less pollution, and can quickly and effectively extract the target substances. The accuracy and precision meet the requirements. It can be used for the detection of six Sudan pigments in chilli powder.