纸片型1-MCP处理对‘朝霞’水蜜桃抗病物质代谢的影响

Effects of paper-containing 1-methylcyclopropene treatment on the disease resistance metabolism of harvested ‘Zhaoxia’ peach fruit

中文关键词:  水蜜桃果实  采后病害  抗病物质代谢  1-甲基环丙烯

英文关键词:peach fruit  postharvest disease  disease resistance metabolism  1-methylcyclopropene

中文摘要:

      目的 研究纸片型1-甲基环丙烯(1-methylcyclopropene, 1-MCP)处理对采后水蜜桃果实抗病物质代谢的影响。方法 ‘朝霞’水蜜桃果实采后通过0(对照组)和1.2 μL/L纸片型1-MCP分别处理12 h, 在(25±1)℃、85%相对湿度下贮藏, 每2 d取样, 评价和测定水蜜桃果实感病指数、抗病相关物质(总酚、木质素)含量及抗病相关酶[过氧化物酶(peroxidase, POD)、多酚氧化酶(polyphenol oxidase, PPO)、几丁质酶(chitinase, CHI)、苯丙氨酸解氨酶(phenylalanine ammonia-lyase, PAL)和β-1,3-葡聚糖酶(β-1,3-glucanase, GLU)]活性。结果 与对照组水蜜桃果实相比, 纸片型1-MCP处理能有效延缓水蜜桃果实采后感病指数的上升, 维持较高的果实果肉木质素和总酚含量, 保持较高的果肉POD、PPO、CHI、PAL和GLU活性。结论 纸片型1-MCP处理可升高水蜜桃果实采后果肉抗病相关酶的活性, 加速果肉累积抗病物质, 进一步提升水蜜桃果实的采后抗病能力, 最终减慢果实采后发生病害及延长果实采后贮藏期。

英文摘要:

      Objective To study the effects of paper-containing 1-methylcyclopropene (1-MCP) treatment on the disease resistance metabolism of harvested peach fruit. Methods The fresh harvested ‘Zhaoxia’ peach fruit was treated by paper-containing 1-MCP at the dosages of 0 (control group) and 1.2 μL/L for 12 h, then stored at (25±1)℃ and relative humidity of 85%. During the storage, the fruit disease index, content of substances related to disease resistance, including total phenolics as well as lignin, and activities of disease resistance-related enzymes, such as peroxidase (POD), polyphenol oxidase (PPO), chitinase (CHI), phenylalanine ammonia-lyase (PAL) and β-1,3-glucanase (GLU) in pulp of peach fruit were assayed every 2 days. Results In contrast to control peach fruit, the paper-containing 1-MCP treatment might effectively delay the increase of fruit disease index of peach fruit during storage. In addition, the paper-containing 1-MCP treatment might maintain the higher content of lignin and total phenolics, and the higher activities of POD, PPO, CHI, PAL and GLU in pulp of peach fruit during storage. Conclusion The paper-containing 1-MCP treatment can improve the disease resistance, suppress the disease development and extend the storage-life of harvested peaches by enhancing the activities of disease resistance-related enzymes and accumulating the amounts of disease resistance-related substances.