3种养殖模式澳洲淡水龙虾肌肉的氨基酸比较分析与评价

Analysis and evaluation of amino acids composition in muscles of Cherax quadricarinatus in 3 kinds of breeding modes

中文关键词:  澳洲淡水龙虾  氨基酸  土池  水泥池  稻田

英文关键词:Cherax quadricarinatus  amino acids  pond  concrete pond  paddy field

中文摘要:

      目的 研究3种养殖模式下澳洲淡水龙虾肌肉中的氨基酸组成差异。方法 通过氨基酸自动分析仪测定土池、水泥池和稻田模式下养殖的澳洲淡水龙虾成虾肌肉中氨基酸含量, 并进行氨基酸品质比较分析。结果 3种养殖模式澳洲淡水龙虾成虾肌肉均含有17种氨基酸, 含量最高的均为谷氨酸, 次之为天门冬氨酸, 最低为胱氨酸, 但17种氨基酸中有11种氨基酸相互呈显著性差异(P<0.05); 3种成虾肌肉中的必需氨基酸与氨基酸总量比值为40.87%~41.35%和必需氨基酸与非必需氨基酸总量比值为69.11%~70.51%; 蛋氨酸+胱氨酸为第一限制性氨基酸; 澳洲淡水龙虾肌肉蛋白与鸡蛋评价模式蛋白较为贴近, 其必需氨基酸指数大小顺序是土池养殖模式(76.47)>稻田养殖模式(72.14)>水泥池养殖模式(71.93)。结论 澳洲淡水龙虾属于优质食物蛋白质, 其氨基酸品质以土池养殖模式为最佳, 稻田次之, 本研究结果为澳洲淡水龙虾养殖及饲料开发等提供理论参考。

英文摘要:

      Objective To study the differences of amino acids composition in muscles of Cherax quadricarinatus in 3 kinds of breeding modes. Methods The content of amino acids in muscles of Cherax quadricarinatus which bred in the pond, concrete pond and paddy field were determined by automatic amino acid analyzer, and the quality of their amino acids were analyzed. Results There were 17 kinds of amino acids in the muscle of adult Cherax quadricarinatus under three culture modes, the highest content was glutamic acid, the second was aspartic acid, and the lowest was cystine acid, but 11 of the 17 kinds of amino acids showed significant differences among each other (P<0.05). The ratios of essential amino acids to total amino acids and essential amino acids to non-essential amino acids in the muscles of the 3 kinds of adult Cherax quadricarinatus were 40.87%?41.35% and 69.11%?70.51%, respectively. Methionine + cystine was the first limiting amino acid. The muscle protein of Cherax quadricarinatus was close to the model protein of egg, and the order of essential amino acid index was pond culture mode (76.47) > paddy field culture mode (72.14) > concrete pond culture mode (71.93). Conclusion Cherax quadricarinatus is a high quality food protein, and its amino acid quality is the best in soil pond culture mode, followed by paddy field culture. The results of this study provide theoretical reference for Cherax quadricarinatus culture and feed development.