杂质残留量对不同栽培方式下灵芝多糖肽定值的影响

Effects of residual impurities on certification of Ganoderma lucidum polysaccharide peptides under different cultivation methods

中文关键词:  灵芝多糖肽  杂质残留量  定值  栽培方式  质量平衡法

英文关键词:Ganoderma lucidum polysaccharide peptides  residual impurities  certification  cultivation methods  mass balance method

中文摘要:

      目的 依据国家标准样品定值要求, 为提高标准样品纯度, 降低杂质残留量, 选择最佳栽培方式的灵芝子实体用于灵芝多糖肽(Ganoderma lucidum polysaccharide peptide, GL-PPSQ2)标准样品的研制。方法 基于高效液相色谱法(high performance liquid chromatography, HPLC), 采用面积归一法对GL-PPSQ2主成分进行定量分析, 卡尔费休库伦法(K-F)测定水分, 热重分析法(thermo gravimetric analysis, TG)测定灰分, 微波消解-电感耦合等离子体质谱法(inductively coupled plasma-mass spectrometry, ICP-MS)测定14种微量元素, 顶空-气相色谱法(headspace-gas chromatography, HS-GC)测量溶剂残留; 质量平衡法对菌草层架(A)、菌草覆土(B)、苡仁壳层架(C)、短段木覆土(D) 4组不同栽培方式所得GL-PPSQ2进行定值。结果 GL-PPSQ2定值依次为: C (97.38%)>A (96.58%)>D (96.02%)>B (95.15%); 得率分别为: B (0.29%)>D (0.18%)>A (0.15%)>C (0.07%); 杂质残留量为: C (1.81%)

英文摘要:

      Objective To select the Ganoderma lucidum fruiting body under the best cultivation conditions for the preparation of Ganoderma lucidum polysaccharide peptide (GL-PPSQ2) for improving the purity and reducing the residual amount of impurities according to the certification of national standards. Methods Area normalization method was used for quantitative analysis of the main component based on high performance liquid chromatography (HPLC), the moisture were determined by Karl Fischer (K-F) method, ash content were determined by thermo gravimetric analysis (TG), 14 kinds of trace elements were determined by microwave digestion-inductively coupled plasma mass spectrometry (ICP-MS) and solvent residue was determined by headspace-gas chromatographic (HS-GC); the certification of GL-PPSQ2 obtained by 4 kinds of different cultivation conditions including Juncao layer frame cultivation (A), Juncao covering soil cultivation (B), adlay husk layer frame cultivation (C) and short-segment wood covering soil cultivation (D) were obtained by mass balance method. Results The purities of GL-PPSQ2 were as follows: C (97.38%)>A (96.58%)>D (96.02%)>B (95.15%); the yields were as follows: B (0.29%)>D (0.18%)>A (0.15%)>C (0.07%); and the residual impurities were as follows: C (1.81%)