超声波法提取淡豆豉总黄酮工艺优化及抗氧化活性研究

Study on optimization of ultrasonic extraction process and antioxidant activity of total flavonoids from Sojae Semen Praeparatum

中文关键词:  淡豆豉  总黄酮  提取工艺  响应面法  抗氧化活性

英文关键词:Sojae Semen Praeparatum  total flavonoids  extraction process  response surface methodology  antioxidant activity

中文摘要:

      目的 优化超声波法提取淡豆豉总黄酮工艺, 并评价淡豆豉总黄酮体外抗氧化活性。方法 以淡豆豉为研究对象, 利用超声波法提取淡豆豉总黄酮, 考察乙醇体积分数、料液比、提取时间对淡豆豉总黄酮含量的影响, 通过响应面实验设计与分析方法, 优化淡豆豉总黄酮提取工艺, 并评价淡豆豉总黄酮体外抗氧化能力。结果 淡豆豉总黄酮最优提取工艺为: 乙醇体积分数40%、料液比1:38 (g/mL)、提取时间60 min, 此条件下总黄酮含量可达(7.967±0.031) mg/g; 淡豆豉总黄酮具有一定抗氧化活性, 其对1,1-二苯基-2-苦基肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)、2,2’-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐[2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, ABTS]、羟自由基(hydroxyl radical, OH·)清除率的半抑制浓度(half maximal inhibitory concentration, IC50)分别为34.88、38.71、452.20 μg/mL。结论 所得到的工艺可以较好提取淡豆豉总黄酮, 淡豆豉总黄酮质量浓度与体外抗氧化活性具有一定的量效关系。

英文摘要:

      Objective To optimize the extraction process of total flavonoids from Sojae Semen Praeparatum by ultrasonic method, and evaluate the in vitro antioxidant activity of total flavonoids from Sojae Semen Praeparatum. Methods The naturally fermented Sojae Semen Praeparatum was taken as the research object, and the effects of the ethanol volume fraction, material-to-liquid ratio, and extraction time on the total flavonoid yield from Sojae Semen Praeparatum were investigated. A Box-Behnken response surface test design and analysis method were used to optimize the production of Sojae Semen Praeparatum. The process for extracting the total flavonoids and the anti-oxidation ability of the total flavonoids in Sojae Semen Praeparatum were evaluated. Results The optimal process for extracting the total flavonoids from Sojae Semen Praeparatum was as follows: Ethanol volume fraction 40%, material-to-ratio liquid 1:38 (g/mL), and extraction time 60 min. Under this process, the total flavonoid yield could reach (7.967±0.031) mg/g. The total flavonoids in Sojae Semen Praeparatum had certain antioxidant activity, and the half maximal inhibitory concentration (IC50) of the scavenging rates for 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and hydroxyl radical (OH·) were 34.88, 38.71 and 452.20 μg/mL, respectively. Conclusion The obtained process can better extract the total flavonoids of Sojae Semen Praeparatum, and the mass concentration of Sojae Semen Praeparatum total flavonoids has a certain dose-effect relationship with the antioxidant activity in vitro.