高效液相色谱-二极管阵列检测器-荧光检测器检测辣条中16种多环芳烃

Determination of 16 kinds of polycyclic aromatic hydrocarbons in spicy strip by high performance liquid chromatography-diode array detector-fluorescence detector

中文关键词:  辣条  高效液相色谱法  多环芳烃  荧光检测器

英文关键词:spicy strip  high performance liquid chromatography  polycyclic aromatic hydrocarbons  fluorescence detector

中文摘要:

      目的 优化辣条基质中多环芳烃(polycyclic aromatic hydrocarbons, PAHs)的检测方法。方法 采用乙腈提取-QuEChERS净化处理的方式, 通过提取溶剂种类和用量的优化, 建立高效液相色谱-二极管阵列检测器-荧光检测器检测辣条中16种多环芳烃的快速分析方法。结果 采用空白基质提取液配制标准曲线溶液, 16种多环芳烃在20~500 ng/mL的范围内线性良好, 各目标物r2范围在0.9989~1.000之间, 检出限(limits of detection, LODs)为0.08~4.00 μg/kg, 定量限(limits of quantitation, LOQs)为0.30~15.00 μg/kg, 实际样品测试中, 20、40和200 μg/kg 3个水平加标回收率为73.09%~111.18%, 相对标准偏差(relative standard deviations, RSDs)为0.73%~4.48%。结论 该方法灵敏度好、回收率高、精密度高, 适用于辣条中16种多环芳烃的分析检测。

英文摘要:

      Objective To optimize the method of determination polycyclic aromatic hydrocarbons (PAHs) in spicy strips. Methods Acetonitrile extraction-QuEChERS purification treatment was adopted, through the extraction of solvent type and dosage optimization, a rapid analysis method was established for the determination of 16 kinds of PAHs in spicy strips by high performance liquid chromatography equipped with both diode array detector and fluorescence detector. Results Standard curve solution was prepared with blank matrix extract, 16 kinds of PAHs showed good linear relationships ranging from 20-500 ng/mL with r2 from 0.9989 to 1.000, the limits of detection (LODs) were 0.08?4.00 μg/kg, the limits of quantitation (LOQs) were 0.30?15.00 μg/kg. In actual sample tests, recoveries ranged from 73.09% to 111.18% at 3 different level of spiked samples of 20, 40 and 200 μg/kg with relative standard deviations (RSDs) ranging from 0.73% to 4.48%. Conclusion The method has good sensitivity, high recovery and precision, and is suitable for the determination of 16 kinds of PAHs in spicy strip.