超声联合弱酸性电解水处理对草鱼鱼片冷藏期间品质及菌群的影响

Effects of ultrasonic combined with slightly acidic electrolyzed water treatment on quality and bacterial flora of Ctenopharyngodon idella fillets during refrigerated storage

中文关键词:  超声  弱酸性电解水  草鱼  冷藏  菌群组成

英文关键词:ultrasound  slightly acidic electrolyzed water  Ctenopharyngodon idella  refrigeration  bacterial flora composition

中文摘要:

      目的 探究超声与弱酸性电解水联合处理对新鲜草鱼鱼片冷藏期间品质变化及菌群的影响。方法 将新鲜草鱼鱼片分别使用40 kHz, 270 W超声(TW40组)、弱酸性电解水(TEW组)、超声联合弱酸性电解水(TEW40组)处理15 min, 以无菌水浸渍处理15 min样品为对照组(TW组)。将处理后样品放入自封袋置于4 ℃冷藏。每天监测样品的菌落总数(the total viable counts, TVC)、总挥发性盐基氮(total volatile base nitrogen, TVBN)值、硫代巴比妥酸(thiobarbituric acid, TBA)值、pH指标变化, 以及测量冷藏末期菌群的组成。综合评价超声与弱酸性电解水单独或联合处理对新鲜草鱼鱼片4 ℃冷藏期间品质变化影响。结果 超声联合弱酸性电解水处理能明显抑制样品贮藏期间菌落总数的增长, 其pH、TVBN值、TBA值上升速度明显低于TW组的样品。通过菌群的分析得出, 超声联合弱酸性电解水能抑制草鱼鱼片优势菌属不动杆菌属以及假单胞菌属的生长繁殖, 且TEW40组冷藏末期微生物群落丰富度和多样性高于TW组。结论 超声联合弱酸性电解水的前处理方式在有效延长冷藏草鱼等水产品货架期方面具有良好的应用潜力。

英文摘要:

      Objective To investigate the effects of combination of ultrasound and slightly acidic electrolyzed water on the quality changes and bacterial flora of fresh Ctenopharyngodon idella fillets during refrigerated storage. Methods Fresh Ctenopharyngodon idella fillets were treated with 40 kHz, 270 W ultrasound (TW40), slightly acidic electrolyzed water (TEW) and ultrasound combined with slightly acidic electrolyzed water (TEW40) for 15 min, and the samples were dipped in sterile water for 15 min as the control group (TW). Fillets treated by the 4 treatments were placed in ziplock bags and stored at 4 ℃. The total viable counts (TVC), pH, total volatile base nitrogen (TVBN) values and thiobarbituric acid (TBA) values were measured daily to evaluate the effects of single treatment or combined treatment on the quality of fresh Ctenopharyngodon idella fillets during storage at 4 ℃. The effects of ultrasound and slightly acidic electrolyzed water treatment alone or in combination on the quality changes of fresh Ctenopharyngodon idella fillets during 4 ℃ storage were evaluated comprehensively. Results Ultrasound combined with slightly acidic electrolyzed significantly inhibited the growth of total bacterial colonies during 4 ℃ storage, and the pH, TVBN values and TBA values increased at a significantly lower rate than those of the samples in the TW group. The analysis of the bacterial flora composition showed that ultrasonication combined with slightly acidic electrolyzed water could inhibit the growth and reproduction of the dominant genera of Ctenopharyngodon idella fillets, Bacillus spp. and Pseudomonas spp. and the abundance and diversity of microbial community at the end of storage were higher in the TEW40 group than in the TW group. Conclusion The pretreatment method of ultrasonic combined with slightly acidic electrolyzed water has good application potential in effectively prolonging the shelf life of aquatic products such as refrigerated Ctenopharyngodon idella fillets, etc..