铅胶体金免疫层析试纸条研制及其在小龙虾中的应用

Development of lead ions immuno-colloidal gold chromatographic test strip and its application in Procambarus clarkii

中文关键词:  铅  小龙虾  胶体金免疫层析试纸条

英文关键词:lead ions  Procambarus clarkii  immuno-colloidal gold chromatographic test strip

中文摘要:

      目的 利用胶体金免疫层析技术研制重金属铅检测试纸条, 并探究其在小龙虾样品中的应用。 方法 使用胶体金和铅单克隆抗体制备免疫探针。优化反应体系, 构建重金属铅免疫层析检测试纸条。验证该试纸条的检出限、特异性、准确性和稳定性, 并对小龙虾样品进行检测。结果 所研制的重金属铅胶体金免疫层析试纸条的检出限为6 ng/mL, 检测时间为15 min。该试纸条可特异性识别铅, 与其他常见重金属无交叉反应。结论 本研究研制的试纸条性能良好, 能够准确判定小龙虾中重金属铅是否超标。

英文摘要:

      Objective To establish a test strip for heavy metal lead ions based on colloidal gold immunochromatography and explore its application in Procambarus clarkii samples. Methods Colloidal gold and anti-lead monoclonal antibody were used to prepare the immune probes. Reaction system was optimized to develop immuno-colloidal gold chromatographic test strip for heavy metal lead ions. The limit of detection, specificity, accuracy and stability of the test strip were verified, and the Procambarus clarkii samples were detected. Results The limit of detection of the developed immuno-colloidal gold chromatographic test strip for heavy metal lead ions was 6 ng/mL, and the detection time was 15 min. The lead ions could be specifically recognized, but there was no cross-reaction to other common heavy metals. Conclusions The test strip developed in this study provides good performance and can accurately determine excessive content of heavy metal lead ions in Procambarus clarkii.