液相色谱-串联质谱法检测茶叶中真菌毒素的研究进展

Research progress on detection of mycotoxins in tea by liquid chromatography-tandem mass spectrometry

中文关键词:  茶叶  真菌毒素  液相色谱-串联质谱法

英文关键词:tea  mycotoxins  liquid chromatography-tandem mass spectrometry

中文摘要:

      茶叶由于富含多酚类、儿茶素和多种矿物质等, 具有降血脂、抗氧化、减肥、抗菌等多重保健功效而备受欢迎, 其质量安全一直广受关注。茶叶在其生产加工和保存过程中有可能被真菌毒素污染, 真菌毒素由于对人和动物具有严重的危害(致癌性)备受关注。因此针对茶叶中毒素残留的高效、高通量检测方法, 成为了目前研究的重点。本文参考国内外相关文献, 对液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry, LC-MS/MS)在茶叶真菌毒素检测领域的研究进展进行了综述; 对不同前处理提取试剂、净化小柱和检测仪器条件的优劣进行了比较; 对该方法用于检测不同品种茶叶时, 其基质效应对结果的影响进行了分析; 对该方法用于茶叶真菌毒素检测的发展进行了展望, 为检测茶叶中真菌毒素检测标准和限量标准的建立提供理论依据。

英文摘要:

      Tea, rich in polyphenols, catechins and a variety of minerals, is popular for its multiple health care effects such as lowering blood lipids, anti-oxidation, weight loss and antibacterial, and its quality and safety has been widely concerned. Tea may be contaminated with mycotoxins during its production, processing and preservation, and mycotoxins have attracted more attention due to their serious harm to humans and animals (carcinogenicity). Therefore, efficient and high-throughput detection methods for toxin residues in tea have become the focus of current reresearch. This paper reviewed the research progress of liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the detection of mycotoxins in tea, compared the advantages and disadvantages of different pre-treatment extraction reagent, purification column and detection instrument conditions; the influence of matrix effect on the results was analyzed when the method was used to detect different kinds of tea; the development of this method for the detection of mycotoxins in tea was prospected, so as to provide theoretical basis for the establishment of the detection standard and limit standard of mycotoxins in tea.