防城金花茶叶片表型多样性及化学成分关联表型性状分析

Analysis of phenotypic diversity and chemical composition associated phenotypic traits of Camellia nitidissima Chi leaves

中文关键词:  防城金花茶  表型性状  遗传多样性  关联分析

英文关键词:Camellia nitidissima Chi  phenotypic traits  genetic diversity  association analysis

中文摘要:

      目的 基于表型性状及化学成分, 分析防城金花茶叶片表型性状遗传多样性, 并采用相关分析法, 筛选与化学成分关联的表型性状。方法 通过叶片表型直接观察法和超高效液相色谱(ultra performance liquid chromatography, UPLC)指纹图谱检测方法, 基于液相色谱峰数量和面积, 采用主成分分析、相关分析方法对防城金花茶遗传多样性、表型性状和化学成分关联性状进行评价。结果 防城金花茶叶片各表型性状遗传多样性程度均较高, 非数值型性状变异系数较高的为叶形(103.570%)、叶色(103.711%); 数值型性状变异系数较高的为叶片总数(54.190%)和产量(57.140%)。每个株系所含的化学成分种类不尽相同, 多样性指数最低的为表儿茶素(0.310), 最高的为19-去甲睾酮(2.068), 相对于其他已知化学成分含量, 马钱苷含量最高。多数化学成分与叶尾和叶脉有显著相关性; 叶面积、叶柄及叶长宽比与极少数成分有相关性, 其中叶尾渐尖、叶脉浅且细脉少、叶面积较大者, 多数化学成分含量较高。结论 防城金花茶叶片遗传多样性丰富, 不同株系化学成分存在较大差异, 可通过叶面积大小、叶尾形状、叶脉深浅、细脉多少筛选多数化学成分含量高的株系。

英文摘要:

      Objective To analyze the genetic diversity of phenotypic traits of Camellia nitidissima Chi leaves based on phenotypic traits and chemical components, and screen the phenotypic traits associated with chemical components by correlation analysis. Methods Leaf phenotypic direct observation and ultra performance liquid chromatography (UPLC) fingerprint detection method were used to evaluate the genetic diversity, phenotypic traits and chemical component related traits of Camellia nitidissima Chi leaves by principal component analysis and correlation analysis based on the numbers and areas of liquid chromatography peaks. Results The degrees of genetic diversity of all phenotypic traits of Camellia nitidissima Chi leaves were large with leaf shape (103.57%) and leaf color (103.711%) having the higher variation coefficients of non-numerical traits and the total number of leaves (54.190%) and yield (57.140%) having higher variation coefficients of numerical characters. Each strain contained different kinds of chemical components, the lowest diversity index was epicatechin (0.310) and the highest was 19-nortestosterone (2.068), compared with other known chemical components, the content of loganin was the highest. Furthermore, most chemical components were significantly correlated with leaf tail and vein; leaf area, petiole and leaf length-width ratio were correlated with a few components, the content of most chemical components were higher in those with middle and tail acuminate, shallow veins and few veinlets, and larger leaf areas. Conclusion Camellia nitidissima Chi leaves are rich in genetic diversity, and there are great differences in chemical components among different strains, most strains with high chemical content can be screened by leaf area, leaf tail shape, leaf vein depth and number of veinlets.