基于GOx/NiO@Au-Pt-PGA/GCE酶传感器检测葡萄糖的研究

Detection of glucose based on GOx/NiO@Au-Pt-PGA/GCE enzyme sensor

中文关键词:  纳米材料  酶  传感器  葡萄糖

英文关键词:nanocomposites  enzyme  sensor  glucose

中文摘要:

      目的 构建GOx/NiO@Au-Pt-PGA/GCE传感器对果蔬中的葡萄糖快速定量的分析方法。方法 在氧化镍纳米材料(nickel oxide nanomaterials, NiO)的基础上复合金-铂纳米复合材料(gold-platinum nanocomposites, Au-Pt)合成新型NiO@Au-Pt纳米复合材料, 以玻碳电极(glassy carbon electrode, GCE)为工作电极, 聚谷氨酸[poly-(γ-glutamic acid), PGA]为交联剂交联葡萄糖氧化酶(glucose oxidase, GOx)和NiO@Au-Pt, 制备GOx/NiO@Au-Pt-PGA/GCE传感器。通过对交联剂浓度、静置时间的优化确定检测体系最佳工作条件。结果 NiO@Au-Pt具有良好的导电性和催化作用, PGA具有良好的生物相容性和协同作用, GOx/NiO@Au-Pt-PGA/GCE能有效提高传感器的灵敏度, 增强电流响应。GOx/NiO@Au-Pt-PGA/GCE的最佳工作条件为1.0% PGA作为制备传感器的交联剂, 静置时间20 s。在最佳条件下, 葡萄糖浓度与其峰电流在0.01~9.00 mmol/L内呈线性关系, Y=4.1858X+0.169, r2=0.9946, 检出限(S/N=3)为2.43 μmol/L。GOx/NiO@Au-Pt-PGA/GCE对梨汁中葡萄糖检测结果与国家标准规定的高效液相色谱法一致, 且GOx/NiO@Au-Pt-PGA/GCE稳定性较好, 4 ℃保存15 d后其峰电流仅降低了4.89%, 对常见的共存干扰物有较好的抗干扰能力。结论 GOx/NiO@Au-Pt-PGA/GCE简单便捷、稳定性好, 可用于果蔬中葡萄糖的快速分析。

英文摘要:

      Objective To establish a GOx/NiO@Au-Pt-PGA/GCE sensor for rapid quantitative analysis of glucose in fruits and vegetables. Methods NiO@Au-Pt nanocomposites were synthesized based on nickel oxide nanomaterials (NiO) by compositing gold-platinum nanocomposites (Au-Pt), GOx/NiO@Au-Pt-PGA/GCE sensor was manufactured using glass carbon electrode (GCE) as working electrode, poly-(γ-glutamic acid) (PGA) as crosslinking agent for crosslinking glucose oxidase (GOx) and NiO@Au-P. The optimum working conditions of the detection system were determined by optimizing the concentration of crosslinking agent and standing time. Results NiO@Au-Pt had good conductivity and catalysis, PGA had good biocompatibility and synergy, GOx/NiO@Au- Pt-PGA/GCE could effectively improve the sensitivity of the sensor and enhance the current response. The optimum working condition of GOx/NiO@Au-Pt-PGA/GCE was that 1.0% PGA as the crosslinker for preparing the sensor and the standing time was 20 s. Under the optimum conditions, there was a linear relationship between glucose concentration and its peak current in the range of 0.01-9.00 mmol/L, Y=4.1858X+0.169, r2=0.9946 and the limit of detection (S/N=3) was 2.43 μmol/L. The detection results of glucose in pear juice using GOx/NiO@Au-Pt-PGA/GCE was consistent with the result of high performance liquid chromatography stipulated by the national standard, and the stability of GOx/NiO@Au-Pt-PGA/GCE was good, the peak current of GOx/NiO@Au-Pt-PGA/GCE decreased only 4.89% after being stored at 4 ℃ for 15 days, and GOx/NiO@Au-Pt-PGA/GCE had good anti-interference ability to common coexistence disturbance. Conclusion GOx/NiO@Au-Pt-PGA/GCE is simple, convenient and stable, and can be used for rapid analysis of glucose in fruits and vegetables.