羊乳高F值肽酶解工艺优化

Optimization of enzymatic hydrolysis of High Fischer Ratio Peptide from goat milk

中文关键词:  羊乳  酶解  高F值肽  工艺优化

英文关键词:goat milk  enzymolysis  High Fischer Ratio Peptide  process optimization

中文摘要:

      目的 探究通过生物酶解技术有效得到羊乳高F值肽的关键工艺。方法 以新鲜山羊乳为原料, 采用两种酶分步水解法制备高F值肽, 以蛋白质水解度和F值为指标, 通过单因素试验和响应面分析法分别确定两步酶解的最佳条件, 酶解液经活性炭静态吸附去除游离芳香族氨基酸, 对脱去芳香族氨基酸后的酶解液进行氨基酸组成分析并测定F值。结果 水解羊乳蛋白最佳内肽酶为碱性蛋白酶, 最优工艺为: 加酶量4000 U/g、底物浓度6%、酶解pH 9.0、温度45 ℃、时间3 h; 最佳外肽酶为风味蛋白酶, 最优工艺为: 加酶量6200 U/g、酶解pH 6.5、温度49 ℃、时间2 h。羊乳经两步酶解及活性炭吸附后, F值可达21.82, 符合高F值肽的产品要求。结论 羊乳高F值肽的酶解工艺研究可为功能性营养食品的开发及陕西羊乳的高值化利用提供技术支撑。

英文摘要:

      Objective To explore the key process for effectively obtaining High Fischer Ratio Peptide from goat milk through biological enzymatic hydrolysis technology. Methods Using fresh goat milk as raw material, the High Fischer Ratio Peptide was prepared by a two-enzyme step-by-step hydrolysis method. Taking the degree of protein hydrolysis and F value as indicators, and the optimal conditions for the two-step enzymatic hydrolysis were determined by single factor and response surface analysis methods. Hydrolysates derived from goat milk protein were static adsorbed by activated carbon in order to remove liberated aromatic amino acids, the amino acid composition and F value of the hydrolysate after dearomatization were analyzed. Results Alkaline protease was selected as the best endopeptidase for hydrolyzing goat milk protein, the optimal processes were as follows: The ratio of enzyme to substrate 4000 U/g, substrate concentration 6%, pH 9.0, enzymatic temperature 45 ℃, and reaction time 3.0 h; flavourzyme was selected as the best exopeptidase enzyme, the optimal process was as follows: The ratio of enzyme to substrate 6200 U/g, enzymolysis pH 6.5, enzymatic temperature 49 ℃, and reaction time 2.0 h. After two-step enzymolysis of goat milk, the F value of the samples adsorbed by activated carbon reached 21.82, and met the product requirements of High Fischer Ratio Peptide. Conclusion The enzymatic hydrolysis process of goat milk High Fischer Ratio Peptide can provide technical support for the development of functional nutritional food and the high value utilization of Shaanxi goat milk.