金黄色葡萄球菌ST398-t571型在陕西生猪屠宰场的流行传播及其耐药性分析

Epidemic spread and drug resistance analysis of Staphylococcus aureus ST398-t571 type in swine slaughterhouse in Shaanxi

中文关键词:  金黄色葡萄球菌  屠宰链  分子分型  毒力基因  耐药性

英文关键词:Staphylococcus aureus  slaughter chain  molecular typing  virulence gene  drug resistance

中文摘要:

      目的 调查陕西省某生猪屠宰场ST398-t571型金黄色葡萄球菌的流行情况及耐药性。方法 本研究对陕西省某生猪屠宰链及屠宰场内部环境共采集485份样品。使用常规检测方法和分子生物学技术对金黄色葡萄球菌进行分离鉴定, 然后对分离株进行分子分型鉴定和对15种抗菌药物进行耐药性试验; 并对其进行26种毒力基因和13种耐药基因的检测。结果 样品中共分离出62株金黄色葡萄球菌, 总分离率为12.8%; 其中ST398-t571型分离株为优势克隆型, 占90.3% (56/62)。耐药试验结果显示, 所有受试菌株均对苯唑西林、阿米卡星、万古霉素和头孢噻肟敏感, 对其余的抗菌药物表现出不同程度的耐药性。26种毒力基因中11种毒力基因(seb、seg、sei、sek、sen、seq、lukD、lukE、lukF-PV、lukS-PV、sasG)被检出。13种被检耐药基因除万古霉素耐药基因vanA基因外均有检出。结论 屠宰场生猪屠宰链普遍存在以ST398-t571型为主的金黄色葡萄球菌污染, 不同批次分离株基因携带情况存在一定差异且菌株存在较为严重的耐药性。

英文摘要:

      Objective To investigate the prevalence status and drug resistance of Staphylococcus aureus ST398-t571 type in swine slaughterhouse in Shaanxi Province. Methods In the current study, 485 samples were collected from a swine slaughter chain and the internal environment of a slaughterhouse in Shaanxi Province. Staphylococcus aureus was isolated and identified by conventional detection methods and molecular biological techniques, and then the isolates were identified by molecular typing and resistance test to 15 kinds of antimicrobial drugs; 26 kinds of virulence genes and 13 kinds of drug resistance genes were detected. Results A total of 62 strains of Staphylococcus aureus were isolated from the samples, and the total isolation rate was 12.8%; among them, the ST398-t571 type isolate was the dominant clonotype 90.3% (56/62). The results of antimicrobial resistance test showed that all tested strains were sensitive to oxacillin, amikacin, vancomycin and cefotaxime, and showed varying degrees of resistance to the rest of the antibiotics. 11 kinds of virulence genes (seb, seg, sei, sek, sen, seq, lukD, lukE, lukF-PV, lukS-PV, sasG) were detected among the 26 kinds of virulence genes. All the 13 detected drug resistance genes were detected except the vancomycin resistance gene vanA gene. Conclusion ST398-t571 type is the main type of Staphylococcus aureus in the swines slaughtering chain, and there are certain differences in the gene carrying status of isolates from different batches and these strains has serious drug resistance.