离子色谱法测定蜂蜜中松二糖含量

Determination of turanose in honey by ion chromatography

中文关键词:  蜂蜜  松二糖  离子色谱法  鉴别

英文关键词:honey  turanose  ion chromatography  identification

中文摘要:

      目的 建立离子色谱法测定蜂蜜中松二糖的检测方法, 初步分析市售蜂蜜松二糖含量情况。方法 用水作为样品提取剂, 以136 mmol/L氢氧化钠溶液和20 mmol/L无水乙酸钠溶液作为淋洗液等度洗脱, 进样量20 μL, 流速0.7 mL/min, 采用Metrosep Carb 2分离柱(250 mm×4.0 mm, 5.0 μm), 以安培检测器进行测定。结果 离子色谱法测定蜂蜜中松二糖相关系数为0.99955, 检出限达0.02 g/100 g, 定量限为0.05 g/100 g, 定量和定性的相对标准偏差分别为1.62%和0.089%, 加标回收率为98.3%。结论 本研究建立的检测蜂蜜中松二糖含量的方法, 前处理简单、分离效果好、灵敏度高、检测结果稳定可靠, 可用于蜂蜜中松二糖含量的检测。通过对84个市购蜂蜜样品中松二糖含量测定分析, 可对蜂蜜中松二糖的含量测定、蜂蜜真伪鉴别及制定蜂蜜相关标准提供技术支持。

英文摘要:

      Objective To establish an ion chromatography method for the determination of turanose in honey, and preliminary analyze the content of turanose in honey sold in the market. Methods Water was used as the sample extractant, and 136 mmol/L sodium hydroxide solution and 20 mmol/L anhydrous sodium acetate solution were used as eluent for isocratic elution, the injection volume was 20 μL and the flow rate was 0.7 mL/min, using a Metrosep Carb 2 separation column (250 mm×4.0 mm, 5.0 μm), the measurements were performed with an ampere detector. Results The correlation coefficient of turanose in honey determined by ion chromatography was 0.99955, the limit of detection was 0.02 g/100 g, and the limit of quantification was 0.05 g/100 g, the relative standard deviations of quantitative and qualitative were 1.62% and 0.089%, respectively, and the recovery rates were 98.3%. Conclusion The method established in this experiment to detect the turanose in honey has simple pre-processing, good separation effect, high sensitivity, stable and reliable detection results, and can be used for the detection of turanose content in honey. Through the determination and analysis of the content of turanose in 84 commercially available honey samples, technical support can be provided for the determination of the content of turanose in honey, the identification of the authenticity of honey, and the formulation of honey-related standards.