不同干燥方式对陈皮中7种黄酮含量及抗氧化性的影响

Effects of drying methods on the content of 7 kinds of flavonoids and antioxidant activity in Citri retriculatae Pericarpium

中文关键词:  陈皮  黄酮  自然晾晒  热风烘干  冷冻干燥  抗氧化性

英文关键词:Citri retriculatae Pericarpium  flavonoids  natural drying  hot-air drying  freeze drying  antioxidant activity

中文摘要:

      目的 考察不同干燥方式对广陈皮(Citri reticulatae pericarpium, CRP)和普通陈皮中7种黄酮含量及抗氧化性的影响。方法 对广东新会茶枝柑和福建芦柑果皮采用3种干燥方式(自然晾晒、热风烘干、冷冻干燥)制备陈皮, 利用高效液相色谱法测试样品中7种黄酮(橙皮苷、香蜂草苷、甜橙黄酮、去甲基川陈皮素、3,5,6,7,8,3’,4’-七甲氧基黄酮、川陈皮素、橘皮素)的含量, 采用二苯苦味酰肼(1,1-diphenyl-2-picrylhydrazyl, DPPH)、2,2’-联氮-二-(3-乙基-苯并噻唑啉-6-磺酸)[2,2’-azinobis (3-ethylbenzothiazoline-6-sulphonic acid), ABTS]、OH自由基清除活性和还原能力测试样品的抗氧化性。结果 对于本研究的两种陈皮, 热风烘干和自然晾晒陈皮的黄酮含量较高; 热风烘干陈皮的抗氧化性最高, 其次是自然晾晒, 且OH自由基清除活性较其他方法更能真实地反映陈皮黄酮的抗氧化性。结论 热风烘干制备陈皮具有较高的黄酮含量和抗氧化性。研究结果可为区分了解不同干燥方式制备陈皮的品质提供依据, 同时为陈皮干燥方式的选择提供参考。

英文摘要:

      Objective To investigate the effects of different drying methods on the content and antioxidant activity of 7 kinds of flavonoids in Guangdong Citri retriculatae Pericarpium (CRP) and ordinary CRP. Methods Citrus reticulata “Chachi” (Guangdong) and Citrus reticulata “Ponkan” (Fujian) were dried in 3 kinds of ways (natural drying, hot-air drying, and freeze drying) to prepare dried tangerine peel, for the dried samples, the content of 7 kinds of flavonoids (hesperidin, didymin, sinensetin, nobiletin, 3,5,6,7,8,3’,4’-heptanmethphoxyflavone, tangeretin, demethylnobiletin) was analyzed by high performance liquid chromatography method, and the antioxidant activity was estimated by the 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), OH radical scavenging activity and reducing power. Results For the 2 kinds of CRP in this study, the flavonoid content in hot-air drying and natural drying CRP were higher, dried CRP with hot-air showed the highest antioxidant activity, followed by natural drying, compared with other methods, the OH radical scavenging activity could more truly reflect the antioxidant activity of flavonoids in CRP. Conclusion The CRP prepared by hot-air drying has high flavonoids content and oxidation resistance. The results can provide a basis for distinguishing the quality of CRP prepared by different drying methods, and also provide a reference for the selection of drying methods of CRP.