膳食鱼油对运动大鼠骨骼肌耗氧量及运动疲劳的影响

Effects of dietary fish oil on skeletal muscle oxygen consumption and exercise fatigue in exercise rats

中文关键词:  多不饱和脂肪酸  耗氧量  运动疲劳

英文关键词:polyunsaturated fatty acids  oxygen consumption  exercise fatigue

中文摘要:

      目的 考察膳食鱼油对运动大鼠骨骼肌耗氧量的影响及抗运动疲劳作用。方法 采用膳食鱼油喂食雄性Wistar大鼠8周, 使用大鼠自体血液灌注后肢, 通过坐骨神经(1 Hz、6~12 V、0.05 ms)刺激骨骼肌, 并测定膜脂肪酸组成、骨骼肌耗氧量、肌肉收缩、收缩间恢复的变化。结果 膳食鱼油饮食组(FO)与对照组(CK)相比显著增加了红白骨骼肌膜中二十二碳六烯酸(docosahexaenoic acid, DHA)、总n-3不饱和脂肪酸(polyunsaturated fatty acids, PUFA)含量, 降低了n-6:n-3 PUFA比值(P<0.05)。CK组和FO组三轮刺激时的初始和最大张力相比均有显著差异(P<0.05), FO组第一轮和第二轮的初始张力和最终张力之间存在显著差异，FO组第一轮和第二轮的最大张力和最终张力相比没有显著降低(P>0.05)。FO组的耗氧量与CK组相比无显著降低(P>0.05), O2效果指数显著高于对照组(P<0.05)。FO组与CK组相比恢复时静息耗氧量有所减少。结论 通过膳食鱼油喂食大鼠发现膳食鱼油中的多不饱和脂肪酸DHA的渗入与增加肌肉氧气消耗效率和促进抵抗肌肉疲劳有关。

英文摘要:

      Objective To investigate the skeletal muscle oxygen consumption and anti-exercise fatigue effects of dietary fish oil on exercise rats. Methods Male Wistar rats were fed dietary fish oil for 8 weeks, the rats in vivo autologous perfused hind limb were used to investigate the influences of membrane composition on skeletal muscle function, hind limb skeletal muscle perfused using the animal’s own blood was stimulated via the sciatic nerve (1 Hz, 6-12 V, 0.05 ms) to contract, and the changes of membrane fatty acid composition, skeletal muscle oxygen consumption, muscle contraction and intersystolic recovery were determined. Results The dietary fish oil diet (FO) significantly increased the docosahexaenoic acid (DHA) and total n-3 polyunsaturated fatty acids (PUFA) content in the red and white skeletal muscle membrane and decreased the n-6:n-3 PUFA ratio (P<0.05) compared with control group (CK). There were significant differences between CK and FO group in initial and maximum twitch tension after three contraction rounds (P<0.05), there were significant differences between the initial and final twitch tension of the first and second contraction rounds of the FO group (P<0.05), there was no significant difference between maximum tension and final tension of the first and second rounds of FO group (P>0.05). Compared with CK, the hind limb oxygen consumption was no significantly reduced in the dietary fish oil group during contraction (P>0.05), and the O2 efficiency index was significantly higher than that of the control group (P<0.05). the FO resting oxygen consumption was increased compared with CK. Conclusion Feeding rats with dietary fish oil find that the infiltration of polyunsaturated fatty acid DHA in dietary fish oil is related to increasing the efficiency of muscle oxygen consumption and promoting the resistance to muscle fatigue.