低温等离子体处理对鲜参切片的杀菌效果及表面色泽的影响

Effects of low temperature plasma treatment on sterilization effects and surface color of fresh-cut ginseng slices

中文关键词:  鲜参切片  低温等离子体  杀菌  保鲜  响应面法

英文关键词:fresh-cut ginseng slices  low temperature plasma  sterilization  preservation  response surface methodology

中文摘要:

      目的 探讨低温等离子体处理对鲜参切片微生物的杀菌效果及色泽的影响, 获得优化杀菌工艺条件。方法 以杀菌率为指标, 考察放电电源功率(W)、等离子体处理时间(min)、气体流速(cm3/min) 3个因素的杀菌效果, 然后根据单因素实验的结果, 应用三因素三水平响应面优化实验设计, 获得最佳杀菌工艺, 并测定低温等离子体处理鲜参切片的色泽变化。结果 低温等离子体处理鲜参切片的最佳杀菌工艺条件为: 放电电源功率340 W、等离子体处理时间4.7 min、气体流速10 cm3/min, 在此条件下的杀菌率为99.89%; 影响因素的显著性大小顺序为: 等离子体处理时间>放电电源功率>气体流速。鲜切参片经低温等离子体处理后L*、a*、b*变化不显著; ΔE*为2.40, 总色差值较小, 说明处理后色泽无明显变化。与酸性氧化电位水杀菌相比, 低温等离子体处理的杀菌率提高了5.3%, 杀菌时间缩短了57%, 杀菌效果更好。结论 低温等离子体处理对鲜参切片微生物的杀菌效果显著, 可以作为一种更高效、更省时的非热杀菌方法。

英文摘要:

      Objective To explore the sterilization effects of low-temperature plasma treatment on microorganisms in fresh ginseng slices and its influence on the color of fresh ginseng slices, and obtain the optimized sterilization process conditions. Methods With the sterilization rate as the index, the sterilization effects of 3 factors of discharge power (W), plasma treatment time (min) and gas flow rate (cm3/min) were investigated. Then, according to the results of single factor experiment, the experimental design was optimized by response surface of 3 factors and 3 levels to obtain the best sterilization process, and the color change of fresh ginseng slices treated by low temperature plasma was measured. Results The optimum sterilization process conditions of low-temperature plasma treatment of fresh ginseng slices were as follows: Discharge power of 340 W, plasma treatment time of 4.7 min and gas flow rate of 10 cm3/min. Under these conditions, the sterilization rate was 99.89%. The order of significance of influencing factors was: Plasma treatment time>discharge power>gas flow rate. Moreover, the changes of L*, a* and b* of fresh-cut ginseng slices after low-temperature plasma treatment were not significant. The ΔE* was 2.40, and the total chromatic aberration value was small, indicating that the color had no obvious change after treatment. Compared with acid oxidation potential water sterilization, the sterilization rate of low-temperature plasma treatment was increased by 5.3%, the sterilization time was shortened by 57%, and the sterilization effect was better. Conclusion Low-temperature plasma treatment has remarkable sterilization effect on microorganisms on the surface of fresh ginseng slices, which can be used as a more efficient and time-saving non-thermal sterilization method.