聚乙烯亚胺纳米银的制备及饮料中亮蓝的检测应用

Preparation of polyethyleneimine silver nanoparticles and their application for detecting brilliant blue in beverage

中文关键词:  纳米银  聚乙烯亚胺  表面增强拉曼散射  亮蓝  抗氧化性

英文关键词:nano silver  polyethyleneimine  surface-enhanced Raman scattering  brilliant blue  oxidation resistance

中文摘要:

      目的 制备一种高稳定性、带正电荷的纳米银(nano silver, AgNPs)用作表面增强拉曼散射(surface-enhanced Raman scattering, SERS)光谱基底材料, 建立饮料中阴离子色素亮蓝的快速检测方法。方法 本研究以硝酸银作为银源、聚乙烯亚胺(polyethyleneimine, PEI)为还原剂, 密封气相顶空瓶作为反应容器, 在160 ℃的油浴中, 利用密封体系亚临界水热法, 仅需10 min即可快速得到形貌、粒径分布均一的聚乙烯亚胺包覆的纳米银(polyethyleneimine coated nano-silver, PEI@AgNPs)。将其通过简单的浸泡-吸附组装至滤纸上制得到三维SERS基底(polyethyleneimine coated nano-silver-three-dimensional SERS surface, PEI@AgNPs-3D)。 结果 该法制备的PEI@AgNPs表面带正电荷, 其Zeta电位高达+44.3 mV, 且具有良好的抗氧化性能, 在空气中存放3个月SERS活性保持不变; PEI@AgNPs-3D成功用于饮料中亮蓝的分析测检测, 检出限为0.022 mg/L, 该方法在亮蓝0.1~10.0 mg/L浓度范围内呈良好的线性关系, 测得实际样品芬达饮料中亮蓝的含量与国家标准方法检测结果基本一致。结论 该法制备的PEI@AgNPs稳定性好, 利用其构建的PEI@AgNPs-3D SERS基底建立了阴离子亮蓝分析方法, 成功用于饮料中亮蓝的检测。

英文摘要:

      Objective To prepare a high stability and positive charged nano silver (AgNPs), which was used as surface-enhanced Raman scattering (SERS) spectral substrate to establish a rapid detection method for anionic pigment brilliant blue in beverage. Methods In this study, silver nitrate was used as silver source, polyethyleneimine (PEI) was used as reducing agent, and sealed gas-phase headspace bottle was used as reaction vessel, the polyethyleneimine coated nano-silver (PEI@AgNPs) with uniform morphology and particle size distribution was quickly prepared through sealing system subcritical hydrothermal method in 160 ℃ oil bath, just needed 10 min. The PEI@AgNPs was assembled on filter paper by simple immersion-adsorption to prepare three-dimensional SERS substrate (PEI@AgNPs-3D). Results The surface of PEI@AgNPs prepared by this method was positively charged, it’s Zeta potential was up to +44.3 mV, and it showed excellent inoxidizability, whose SERS activity can keep unchanged for 3 months in air; the PEI@AgNPs-3D was successfully used for the analysis and detection of brilliant blue in beverage, the limit of detection was 0.022 mg/L and this method showed a good linear relationship in the concentration range of brilliant blue between 0.1?10.0 mg/L, and the detection result of brilliant blue in the actual sample fanta beverage was basically consistent with that of the national standard method. Conclusion The PEI@AgNPs which is prepared by this method has high stability, the anionic bright blue analysis method is established by using the PEI@AgNPs-3D SERS substrate constructed by PEI@AgNPs, and successfully applies to the detection of bright blue in beverages