有机溶剂溶解法结合电感耦合等离子体发射光谱法直接测定软胶囊剂型保健食品中的钙和铁

Direct determination of calcium and iron in soft capsule health food by organic solvent dissolution combined with inductively coupled plasma optical emission spectrometry

中文关键词:  软胶囊剂型保健食品  钙  铁  二甲亚砜  直接进样  电感耦合等离子体发射光谱法

英文关键词:soft capsule health food  calcium  iron  dimethyl sulfoxide  direct test  inductively coupled plasma optical emission spectrometry

中文摘要:

      目的 建立电感耦合等离子体发射光谱法(inductively coupled plasma optical emission spectrometry, ICP-OES)测定软胶囊剂型保健食品中的钙和铁含量的方法。方法 软胶囊剂型保健食品经水-硝酸-二甲亚砜混合溶液溶解后, 使用电感耦合等离子体发射光谱仪, 直接测定其中的钙和铁含量。结果 钙元素方法的检出限为3 mg/kg, 定量限为9 mg/kg, 钙元素的回收率在92.2%~96.7%之间, 相对标准偏差(relative standard deviations, RSDs)在3.2%~8.8%之间。铁元素方法的检出限为0.6 mg/kg, 定量限为2 mg/kg, 铁元素的回收率在91.8%~96.7%之间, RSDs在1.8%~11.9%之间。实际样品检测时, 同时采用了本方法与GB 5009.268—2016《食品安全国家标准 食品中多元素的测定》(第二法), 两种方法所得结果没有明显差异。结论 本方法在前处理时, 不再使用传统的微波消解等方法, 操作简单、快捷、准确度高, 可以用于软胶囊剂型保健食品中钙和铁的检测。

英文摘要:

      Objective To establish a method of the determination of calcium and iron in soft capsules health food by inductively coupled plasma optical emission spectrometry (ICP-OES). Methods After the health food in the form of soft capsules was dissolved in the mixed solution of water, nitric acid and dimethyl sulfoxide, the content of calcium and iron in the food were directly determined by inductively coupled plasma atomic emission spectrometry. Results The limits of detection and limits of quantitation of the calcium method were 3 mg/kg and 9 mg/kg, respectively, the recoveries of calcium were 92.2%?96.7% and the relative standard deviations (RSDs) were 3.2%?8.8%. The limits of detection and limits of quantitation of the iron method were 0.6 mg/kg and 2 mg/kg, respectively, the recoveries of iron were within the range of 91.8%?96.7% and RSDs were within the range of 1.8%?11.9%. This method and GB 5009.268—2016 National food safety standards-Determination of multi-elements in food (the second method) were simultaneously used for the actual sample detection, and the results obtained by the 2 kinds of methods were not significantly different. Conclusion In the pretreatment of the method, the traditional methods such as microwave digestion are not used, the operation is simple, rapid and high in accuracy, and the method can be used for detecting calcium and iron in soft capsule health food.