荷叶生物活性效应标志成分分析

Analysis of biomarker components of lotus leaf bioactivity effect

中文关键词:  荷叶  生物效应成分  荷叶碱  2-羟基-1-甲氧基阿朴啡

英文关键词:lotus leaf  biomarker components  nuciferine  2-hydroxy-1-methoxy-laporphine

中文摘要:

      目的 筛选荷叶生物活性效应标志成分, 为荷叶质量控制提供参考。方法 采用高效液相色谱法同时测定荷叶中8种黄酮或生物碱成分含量, 并对荷叶抗氧化、抑制脂肪酶活性效应与成分含量相关性进行分析。采用薄层色谱法分离分析荷叶特征色谱斑点, 聚类分析法筛选质量标志成分。结果 8种成分含量在0.61~9.93 mg/g之间; 荷叶碱、N-去甲基荷叶碱和2-羟基-1-甲氧基阿朴啡可作为荷叶化学质量评价的备选标志成分; 抗氧化活性与金丝桃苷、荷叶碱、2-羟基-1-甲氧基阿朴啡、N-去甲基荷叶碱含量呈现正相关; 脂肪酶抑制率与N-去甲基荷叶碱、金丝桃苷、2-羟基-1-甲氧基阿朴啡、荷叶碱呈现正相关; 薄层色谱法鉴别特征成分为2-羟基-1-甲氧基阿朴啡、荷叶碱。结论 本研究确定荷叶碱、N-去甲基荷叶碱、2-羟基-1-甲氧基阿朴啡和金丝桃苷为荷叶抗氧化、抑制脂肪酶活性效应和化学质量评价指标成分, 有助于全面评价荷叶药材质量及药效活性作用。

英文摘要:

      Objective To screen the bioactive effect marker components of lotus leaf and provide reference for the quality control of lotus leaf. Methods The content of 8 kinds of flavonoids or alkaloids in lotus leaves were determined by high performance liquid chromatography, and the correlations between the antioxidative and lipase inhibitory effects of lotus leaf and the content of the components were analyzed. The characteristic chromatographic spots of lotus leaf were separated and analyzed by thin-layer chromatography, and the quality marker components were screened by cluster analysis. Results The content of 8 kinds of components ranged from 0.61 to 9.93 mg/g. Nuciferine, N-demethyllotine and 2-hydroxy-1-methoxy-apomorphine could be used as alternative markers for chemical quality evaluation of lotus leaf. The antioxidant activity was positively correlated with the content of hyperoside, nuciferine, 2-hydroxy-1-methoxyapomorphine and N-demethyllotine, the inhibition rate of lipase was positively correlated with N-demethyllotine, hyperin, 2-hydroxy-1-methoxy-apomorphine and nuciferine. The characteristic components of thin layer chromatography were 2-hydroxy-1-methoxy-lapomorphine and nuciferine. Conclusion In this study, nuciferine, N-demethyllotine, 2-hydroxy-1-methoxyapophine and hypericin were identified as the antioxidant, lipase inhibitory effect and chemical quality evaluation index components of lotus leaf, which will help to comprehensively evaluate the quality and efficacy of lotus leaf medicinal materials.