液相色谱-质谱法检测保健食品中白藜芦醇及白藜芦醇苷

Determination of resveratrol and resveratrol glucoside in health food by liquid chromatography-mass spectrometry

中文关键词:  白藜芦醇  白藜芦醇苷  液相色谱-质谱法  保健食品

英文关键词:resveratrol  resveratrol glycoside  liquid chromatography-mass spectrometry  health food

中文摘要:

      目的 建立液相色谱-质谱法(liquid chromatography-mass spectrometry, LC-MS)分析保健食品中白藜芦醇及白藜芦醇苷顺反结构(顺式白藜芦醇、反式白藜芦醇、顺式白藜芦醇苷、反式白藜芦醇苷)含量的分析方法。方法 保健食品经80%乙醇于低温条件下避光提取, 经液相色谱柱分离后, 在多反应监测(multiple response monitoring, MRM)模式下进行白藜芦醇及白藜芦醇苷顺反结构含量的测定, 外标法定量。结果 白藜芦醇及白藜芦醇苷顺反结构在5~200 μg/L范围内呈现良好线性关系, 相关系数均大于0.999, 方法检出限为0.02~1.00 μg/L, 定量限为0.06~3.00 μg/L, 平均回收率为94.4%~115.7%, 相对标准偏差为0.8%~7.6% (n=6)。结论 该方法针对保健食品基质, 具有保护白藜芦醇及白藜芦醇苷结构的特点, 操作简便且回收率高, 满足保健食品中白藜芦醇及白藜芦醇苷顺反结构含量的检测要求。

英文摘要:

      Objective To establish an analytical method for analyzing the content of resveratrol and resveratrol glucoside cis-trans structures (cis-resveratrol, trans-resveratrol, cis-resveratrol glucoside and trans-resveratrol glucoside) in health food by liquid chromatography-mass spectrometry. Methods The health food was extracted in the dark with 80% ethanol at low temperature, and separated by liquid chromatography column, the cis-trans structures of resveratrol and resveratrol glucoside were determined in multiple response monitoring (MRM) mode, and quantified by external standard method. Results The cis-trans structure of resveratrol and resveratrol glucoside exhibited good linear relationship within that range of 5-200 μg/L, with the correlation coefficient greater than 0.999, the method detection limits were 0.02-1.00 μg/L, the quantitation limits were 0.06-3.00 μg/L, the average recoveries were 94.4%-115.7%, and the relative standard deviations were 0.8%-7.6% (n=6). Conclusion This method is aimed at the health food substrate, has the characteristics of protecting the structure of resveratrol and resveratrol glycoside, is simple and convenient to operate, has high recovery rate, and meets the detection requirements of the cis-trans structure content of resveratrol and resveratrol glycoside in health food.