虾夷扇贝柱粗多糖不同提取工艺优化及其化学性质与抗氧化活性的比较

Optimization of different extraction processes of crude polysaccharide from Patinopecten yessoensis adductor and comparison of its chemical properties and antioxidant activity

中文关键词:  虾夷扇贝  粗多糖  提取方法  化学性质  抗氧化活性

英文关键词:Patinopecten yessoensis  crude polysaccharide  extraction method  chemical properties  antioxidant activity

中文摘要:

      目的 优化虾夷扇贝柱粗多糖2种提取工艺, 并对比分析2种提取方式对扇贝柱粗多糖的化学性质及抗氧化活性的影响。方法 以提取率为指标, 在单因素实验基础上, 通过响应面分析法对扇贝柱粗多糖热水浸提法及酶解提取法进行优化, 并比较2种粗多糖红外光谱、单糖组成及抗氧化活性。结果 热水浸提法的最优工艺为: 料液比1:3 (g/mL)、浸提时间6 h、浸提温度90 ℃; 酶解法最优工艺为: 木瓜蛋白酶添加量0.3%(鲜重)、pH 7.0、酶解温度55 ℃、酶解时间5 h、料液比1:3 (g/mL)。2种粗多糖组成单糖相同, 但各单糖物质的量之比有所不同; 红外光谱结果表明, 2种粗多糖结构相似, 主要由α-D型吡喃糖构成, 且含有糖醛酸。抗氧化结果表明, 不同提取方式得到的扇贝柱粗多糖具有不同的抗氧化活性, 酶解法提取的粗多糖具有较高的抗氧化活性。结论 本研究确定了虾夷扇贝柱粗多糖2种提取方式的最优条件, 酶解法粗多糖提取率较高且具有较好抗氧化活性, 为后续研究提供了理论依据。

英文摘要:

      Objective To optimize 2 kinds of extraction processes for crude polysaccharide from Patinopecten yessoensis adductor, and compare and analyze the effects of 2 kinds of extraction methods on chemical properties and antioxidant activity of crude polysaccharide from P. yessoensis adductor. Methods With the extraction rate as the index, on the basis of single factor experiment, the hot water extraction and enzymatic extraction of crude polysaccharide from P. yessoensis adductor were optimized by response surface methodology, and the infrared spectra, monosaccharide composition and antioxidant activity of the 2 kinds of crude polysaccharides were compared. Results The optimal conditions of hot water extraction were as follows: Solid-liquid ratio 1:3 (g/mL), extraction time 6 h, and extraction temperature 90 ℃; the optimal conditions for enzymatic hydrolysis were as follows: Papain 0.3% (fresh weight), pH 7.0, enzymolysis temperature 55 ℃, enzymolysis time 5 h and solid-liquid ratio 1:3 (g/mL). The 2 kinds of crude polysaccharides had the same monosaccharide composition, but the amount ratio of each monosaccharide substance was different; the infrared spectrum results showed that the 2 kinds of crude polysaccharides had similar structures, which were mainly composed of α-D pyranose and contained uronic acid. The antioxidant results showed that the crude polysaccharides extracted by different methods had different antioxidant activities, and the crude polysaccharides extracted by enzymatic hydrolysis had higher antioxidant activity. Conclusion This study determines the optimal conditions for the 2 kinds of extraction methods of crude polysaccharide from P. yessoensis adductor, the enzymatic hydrolysis of crude polysaccharide shows high extraction rate and good antioxidant activity, which provides a theoretical basis for the follow-up study.