高效液相色谱法同时测定红美人柑橘不同组织中12种类黄酮化合物

Simultaneous determination of 12 kinds of flavonoids in different parts of ‘Hongmeiren’ citrus by high performance liquid chromatography

中文关键词:  高效液相色谱法  红美人  类黄酮化合物  不同组织

英文关键词:high performance liquid chromatography  ‘Hongmeiren’ citrus  flavonoids compounds  different parts

中文摘要:

      目的 建立一种同时测定红美人柑橘中12种类黄酮化合物(圣草次苷、芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、野漆树苷、香蜂草苷、枸橘苷、柚皮素、橙皮素、川皮苷和橘皮素)的高效液相色谱方法。方法 样品采用甲醇水溶液超声提取, 采用SunFire C18色谱柱(250 mm×4.6 mm, 5 μm)分离, 甲醇-水(含0.2%乙酸)体系作为流动相, 梯度洗脱, 287 nm波长下检测分析。结果 12种类黄酮化合物在各自相应的范围内线性关系良好, 相关系数(r)均不小于0.9998, 12种类黄酮化合物的检出限为0.08~0.50 μg/mL, 3个浓度添加水平的加标回收率范围为90.25%~110.80%, 相对标准偏差(relative standard deviations, RSDs)为1.69%~3.70%。结论 该方法准确度和精密度较高, 简便快速, 适用于红美人柑橘中类黄酮化合物的快速检测。红美人柑橘中的类黄酮化合物主要以橙皮苷、芸香柚皮苷和香蜂草苷为主, 其在果皮和果肉中的含量存在明显差异。

英文摘要:

      Objective To develop a method for the simultaneous determination of 12 kinds of flavonoids (eriocitrin, narirutin, naringin, hesperidin, neohesperidin, rhoifolin, didymin, poncirin, naringenin, hesperetin, nobiletin and tangeretin) in ‘Hongmeiren’ citrus by high performance liquid chromatography. Methods The sample were untrasonically extracted with methanol solution, separated by SunFire C18 column (250 mm×4.6 mm, 5 μm) using methanol and 0.2% acetic acid aqueous solution as mobile phases by gradient elution, and detected at 287 nm. Results All 12 kinds of flavonoids compounds had good linearity within their respective ranges, the correlation coefficients (r) were not less than 0.9998, the limits of detection of 12 kinds of flavonoids were in the range of 0.08–0.50 μg/mL, the spiked recoveries were 90.25%–110.80% at 3 concentration added levels, and the relative standard deviations (RSDs) were in the range of 1.69%–3.70%. Conclusion This method has high accuracy and precision, and is simple and rapid, which is suitable for simultaneous detection of flavonoids compounds in ‘Hongmeiren’. The main flavonoid compounds in ‘Hongmeiren’ citrus are hesperidin, naringin and didymin, and the amounts and distributions have significant differences in the peel and pulp.