超声波辅助提取、纯化滇橄榄多糖的工艺研究

Study on process of ultrasonic assisted extraction and purification of polysaccharides from Phyllanthus emblica Linn.

中文关键词:  滇橄榄  多糖  提取  纯化  工艺优化

英文关键词:Phyllanthus emblica Linn.  polysaccharides  extraction  purification  optimization of process

中文摘要:

      目的 采用超声波辅助法提取滇橄榄多糖, 并采用大孔树脂和透析法对多糖进行除色素纯化。方法 通过单因素实验和正交实验优化滇橄榄多糖的提取工艺及脱色纯化工艺。结果 超声波辅助法提取滇橄榄多糖的最佳条件为: 超声功率为180 W、超声温度为50 ℃、超声时间为45 min、料液比为1:50 (m:V), 得率为6.18%; X-5大孔树脂对滇橄榄多糖脱色条件为: 5 mg/mL滇橄榄多糖溶液、温度为40 ℃、树脂用量为1 g、时间为1 h、pH为1, 色素清除率为82.21%, 多糖保留率为94.86%; 纯化后滇橄榄多糖的纯度由31%提高至91%。结论 本研究得到了滇橄榄多糖提取、纯化的最优条件, 为后续研究及开发利用提供了理论基础。

英文摘要:

      Objective To extract polysaccharides from Phyllanthus emblica Linn. by ultrasonic assisted method, and purify the polysaccharides by macroporous resin and dialysis. Methods Single factor experiment and orthogonal experiment were used to optimize the extraction process, decolorization and purification process. Results The optimum conditions of Phyllanthus emblica Linn. polysaccharides were as follows: Ultrasonic power 180 W, ultrasonic temperature 50 ℃, ultrasonic time 45 min, solid-liquid ratio 1:50 (m:V), and the yield was 6.18%. The decolorization conditions of Phyllanthus emblica Linn. polysaccharides by X-5 macroporous resin were as follows: Five mg/mL Phyllanthus emblica Linn. polysaccharides solution, temperature 40 ℃, resin dosage 1 g, time 1 h, pH 1, pigment removal rate was 82.21%, polysaccharides retention rate was 94.86%; after purification, the purity of Phyllanthus emblica Linn. polysaccharides was increased from 31% to 91%. Conclusion The optimal extraction and purification conditions of polysaccharides from Phyllanthus emblica Linn. are obtained, which provide theoretical basis for further research, development and utilization.