气相色谱-串联质谱法测定植物源性运动食品中肌醇含量

Determination of inositol content in plant-derived sports foods by gas chromatography-tandem mass spectrometry

中文关键词:  植物源性运动食品  QuEChERS  气相色谱-串联质谱法  肌醇

英文关键词:plant-derived sports food  QuEChERS  gas chromatography-tandem mass spectrometry  inositol

中文摘要:

      目的 建立气相色谱-串联质谱法(gas chromatography-tandem mass spectrometry, GC-MS/MS)测定植物源性运动食品中肌醇含量的分析方法。方法 样品经纯水提取后加入3.0 mL硅烷化试剂(V三甲基氯硅烷:V六甲基二硅胺烷=1:3, 现配现用)于85 ℃水浴中衍生75 min; 衍生溶剂经PestiCarb/NH2固相萃取小柱由洗脱溶剂正己烷-丙酮(6:4, V:V)混合溶剂洗脱净化浓缩后经仪器测定。结果 植物源性运动食品中肌醇在质量浓度为0~5.0 μg/mL范围内线性关系良好, 相关系数(r)为0.999, 在不同基质中方法检出限为0.81~1.01 mg/100 g、方法定量限为2.69~3.11 mg/100 g, 加标平均回收率为81.67%~97.45%, 相对标准偏差为1.23%~8.56% (n=6)。结论 该方法前处理简单便捷、灵敏度高、回收率稳定、定量准确, 能快速检测样品中肌醇含量, 完全适用于不同基质的植物源性运动食品中肌醇含量的测定。

英文摘要:

      Objective To establish a method for the determination of inositol content in plant-derived sports foods by gas chromatography-tandem mass spectrometry. Methods The sample was extracted with pure water by 3.0 mL silanization reagent (Vtrimethylchlorosilane:Vhexamethyldisilazane=1:3, ready to use) derivatize in a water bath at 85 ℃ for 75 min; the derivatized solvent was passed through a PestiCarb/NH2 solid phase extraction cartridge by the elution solvent n-hexane-acetone (6:4, V:V). The mixed solvent was eluted, purified and concentrated, and then measured by the instrument. Results The results showed that the inositol in plant-derived sports food had a good linear relationship within the mass concentration range of 0?5.0 μg/mL, the correlation coefficient (r) was 0.999, and the limits of detection of the method in different matrices were 0.81?1.01 mg/100 g, the limits of quantification of the method were 2.69?3.11 mg/100 g, which met the demand of trace detection, the average recoveries of standard addition were 81.67%?97.45%, and the relative standard deviations were 1.23%?8.56% (n=6). Conclusion The method is simple and convenient for pretreatment, high sensitivity, stable recovery, and accurate quantification. It can quickly detect the content of inositol in samples and is completely suitable for the determination of inositol content in plant-derived sports foods with different substrates.