枸杞-沙棘-青稞粉复合物对小鼠的免疫调节功效

Immune regulation efficacy of wolfberry-sea buckthorn-highland barley powder complex on mice

中文关键词:  枸杞  沙棘  免疫调节  体液免疫  细胞免疫

英文关键词:wolfberry  sea buckthorn  immunomodulatory  humoral immunity  cellular immunity

中文摘要:

      目的 探究枸杞沙棘的提取物与青稞粉复合制成的枸杞-沙棘-青稞粉复合物(wolfberry-sea buckthorn-highland barley powder complex, WSHC)对小鼠的免疫调节活性。方法 以斯普拉格-杜勒(Sprague-Dawley, SD)大鼠和昆明种小鼠为研究对象。在对WSHC进行安全性毒理学评估的基础上, 考察WSHC高、中、低3个不同剂量[7.5、5.0、2.5 g/(kg·BW)]对小鼠胸腺脾脏指数(比重法)、细胞免疫[小鼠迟发型变态反应(delayed type hypersensitivity, DTH)和ConA (concanavalin A)诱导的小鼠脾淋巴细胞转化试验、体液免疫(血清溶血素和抗体生成细胞检测)、单核-巨噬细胞功能(小鼠碳廓清试验和小鼠腹腔巨噬细胞吞噬鸡红细胞试验)以及自然杀伤细胞(natural killer cell, NK)功能(乳酸脱氢酶法)]的影响。结果 高剂量WSHC组处理可以显著提高小鼠的细胞免疫, 其中与阴性对照组比, 淋巴细胞增殖能力提高了42.74% (P<0.05), 且小鼠足跖厚度差值提高了47.57% (P<0.01); 中、高剂量的WSHC处理可以显著提高小鼠的体液免疫, 与阴性对照组相比, 中、高剂量WSHC处理溶血空斑数含量分别提高了16.25% (P<0.05)和19.48% (P<0.01), 高剂量WSHC促进小鼠抗体积数提高14.41% (P<0.01); 但各剂量组对免疫器官、单核-巨噬细胞功能、NK细胞功能无明显影响。结论 WSHC可显著提高小鼠淋巴细胞增殖能力和小鼠体液免疫能力, 是一种具有良好开发前景的免疫调节剂。

英文摘要:

      Objective To investigate the immunomodulatory activity of the wolfberry-sea buckthorn-highland barley powder complex (WSHC) made from extracts of wolfberry-sea buckthorn and highland barley flour on mice. Methods Sprague-dawley (SD) rats and Kunming mice were selected as the research objects. Based on the toxicological safety assessment of WSHC, the effects of high, medium and low doses of WSHC [7.5, 5.0, 2.5 g/(kg·BW)] on thymus spleen index (specific gravity method) and cellular immunity [including delayed type hypersensitivity (DTH) and concanavalin A (ConA)-induced mouse spleen lymphocyte transformation test, humoral immunity (serum hemolysin and antibody-producing cells detection), monocyte-macrophage function (mouse carbon clearance test and mouse peritoneal macrophage phagocytic chicken red blood cell test), and natural killer cell (NK) function (lactate dehydrogenase method)] of mice were investigated. Results The high-dose WSHC treatment could significantly improve the cellular immunity function compared with the negative control group, the lymphocyte proliferation ability increased by 42.74% (P<0.05), and the mouse foot plantar thickness difference increased by 47.57% (P<0.01); medium and high doses of WSHC treatment could significantly improve the humoral immunity of mice compared with the negative control group, the number of hemolytic plaques in the medium and high doses of WSHC treatment groups increased by 16.25% (P<0.05) and 19.48% (P<0.01), respectively, high dose WSHC could increase the antibody-producing cells by 14.41% (P<0.01), however, there were no significant effects on immune organs, monocyte-macrophage function, and NK cell function in each dose group. Conclusion WSHC can significantly improve the proliferation of lymphocyte and humoral immunity of mice, which is an immune modulator with good development prospect.