萝卜中戊唑醇的残留状况及膳食风险评估

Residue status and dietary risk assessment of tebuconazole in radish

中文关键词:  萝卜  戊唑醇  超高效液相色谱-串联质谱法  残留  膳食风险

英文关键词:radish  tebuconazole  ultra performance liquid chromatography-tandem mass spectrometry  residue  dietary risk

中文摘要:

      目的 探究戊唑醇在萝卜中的残留状况并评估其膳食风险。方法 样品中残留的戊唑醇采用乙腈提取后, 经N-丙基乙二胺和无水硫酸镁净化, 超高效液相色谱-串联质谱法检测, 基质匹配外标法定量。结果 在0.0005~0.5000 mg/L浓度范围内线性关系良好, 戊唑醇在萝卜根茎和萝卜叶上的线性方程分别为Y=1727069X+21681和Y=524606X+3142, 相关系数(r)大于0.99; 戊唑醇在萝卜根茎和萝卜叶中添加浓度分别为0.010~1.000 mg/kg和0.010~10.000 mg/kg时, 平均回收率分别为76%~96%和93%~109%, 最大相对标准偏差为9.9%, 方法定量限(limits of quantification, LOQs)均为0.010 mg/kg; 在以1.5倍推荐剂量施药后, 戊唑醇在浙江、广西萝卜叶上的平均初始残留量分别为4.6、7.1 mg/kg, 半衰期分别为6.1、4.6 d; 以推荐剂量施药后, 间隔7、14、21 d采集的萝卜根茎和萝卜叶中戊唑醇的残留量分别为<0.01~0.59 mg/kg和0.14~6.25 mg/kg, 推荐安全间隔期(14 d)的戊唑醇国家估算每日摄入量(national estimated daily intake, NEDI)为0.51 mg, 风险概率(risk probability, RQ)为27.0%。结论 虽然戊唑醇在萝卜叶上初始残留量较高但消解迅速, 萝卜根茎及萝卜叶上的最终残留量均未超过我国已制定的最大残留限量, 对一般人群产生的膳食风险可接受。

英文摘要:

      Objective To explore the residue status of tebuconazole in radish and evaluate its dietary risk. Methods The residual tebuconazole in the sample was extracted with acetonitrile, purified with N-propyl ethylenediamine and anhydrous magnesium sulfate, detected by ultra performance liquid chromatography-tandem mass spectrometry, and quantified by matrix matching external standard method. Results The linear relationships were good in the concentration range of 0.0005?0.5000 mg/L, the linear equations of tebuconazole on radish roots and radish leaves were Y=1727069X+21681 and Y=524606X+3142, respectively, and the correlation coefficients (r) were greater than 0.99; when the additive concentrations of tebuconazole in radish roots and radish leaves were 0.010?1.000 mg/kg and 0.010?10.000 mg/kg, respectively, the average recoveries were 76%?96% and 93%?109%, the maximum relative standard deviation was 9.9%, and the limits of quantification (LOQs) of method were 0.010 mg/kg; after 1.5 times the recommended dose, the average initial residues of tebuconazole on radish leaves in Zhejiang and Guangxi were 4.6 and 7.1 mg/kg, respectively, and the half-life was 6.1 and 4.6 days, respectively; after applying the recommended dose, the residues of tebuconazole in radish roots and radish leaves collected at intervals of 7, 14 and 21 days were <0.01?0.59 mg/kg and 0.14?6.25 mg/kg, respectively, the national estimated daily intake (NEDI) of tebuconazole in the recommended safety interval (14 days) was 0.51 mg, and the risk probability (RQ) was 27.0%. Conclusion Although the initial residue of tebuconazole on radish leaves is high, it is digested rapidly. The final residues on radish roots and radish leaves do not exceed the maximum residue limit established in China, and the dietary risk to the general population is acceptable.