低共熔溶剂-超高效液相色谱-串联质谱法快速测定肉制品、水产品中3种非法添加工业染料

Determination of 3 kinds of illegally added industrial dyes in meat and aquatic products by hydrophobicity deep eutectic solvent-ultra performance liquid chromatography-tandem mass spectrometry

中文关键词:  低共熔溶剂  非法添加工业染料  绿色溶剂  超高效液相色谱-串联质谱法

英文关键词:deep eutectic solvent  illegally added industrial dyes  green solvent  ultra performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立低共熔溶剂(deep eutectic solvent, DES)-超高效液相色谱-串联质谱法(ultra performance liquid chromatography-tandem mass spectrometry, UPLC-MS/MS)快速检测肉制品、水产品中3种非法添加工业染料(刚果红、酸性金黄、红2G)的分析方法。方法 样品经水稀释, 微量DES分散萃取, 乙腈复溶。三重四极杆电喷雾离子源电离-多反应监测(multiple reaction monitoring, MRM)-负离子监测模式检测, 外标法定量。结果 刚果红和红2G在0.2~20.0 μg/kg范围内、酸性金黄在0.02~2.00 μg/kg 范围内线性关系良好, 相关系数均大于0.99。方法的检出限分别为0.12、0.26、0.01 μg/kg, 回收率为93.25%~101.36%, 相对标准偏差为1.27%~4.06%。结论 低共熔溶剂对刚果红、酸性金黄、红2G的萃取效果佳, 可以解决传统操作步骤烦琐的问题。本方法快速、简便、精准, 适用于肉制品、水产品中3种非法添加工业染料刚果红、酸性金黄、红2G的检测。

英文摘要:

      Objective To establish a rapid method for determination of 3 kinds of illegally added industrial dyes (Congo red, methaniol yellow, red 2G) in meat and aquatic product by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with deep euectic solvent (DES). Methods The samples were dissolved by water, dispersion extracted by microscale deep euectic solvent, and dissoluted by acetonitrile. Triple quadrupole electrospray ion source ionization-multiple reaction monitoring (MRM)-anion monitoring mode was used for detection, external standard method was used for quantitative analysis. Results The linear relationships of Congo red, red 2G in the ranges of 0.2?20.0 μg/kg and metanil yellow in the ranges of 0.02?2.00 μg/kg were good, respectively, and the correlation coefficients were greater than 0.99. The limits of detection of the method were 0.12, 0.26 and 0.01 μg/kg, respectively. The recovery rates were 93.25%?101.36%, and relative standard deviations were 1.27%?4.06%. Conclusion DES has a good extraction effect of Congo red, red 2G and metanil yellow, which can solve the problem of complicated tradittional operation steps. This method is rapid, simple and accurate, and is suitable for determination of 3 kinds of illegally added industrial dyes in meat and aquatic products.