QuEChERS-表面增强拉曼光谱法测定花生中黄曲霉毒素B1

Determination of aflatoxin B1 in peanut by QuEChERS-surface-enhanced Raman spectroscopy

中文关键词:  黄曲霉毒素B1  表面增强拉曼光谱法  QuEChERS  花生

英文关键词:aflatoxin B1  surface-enhanced Raman spectroscopy  QuEChERS  peanut

中文摘要:

      目的 建立基于QuEChERS净化和表面增强拉曼光谱法(surface-enhanced Raman spectroscopy, SERS)测定花生中黄曲霉毒素B1 (aflatoxin B1, AFB1)的分析方法。方法 以纳米银(nano silver, AgNPs)作为SERS活性基底, 结合QuEChERS样品预处理技术和基于SERS特征峰的非标记检测方法测定花生中的AFB1, 考察凝聚剂种类与浓度、纳米银与样品的比例以及净化剂种类与浓度等条件对实验结果的影响。结果 在凝聚剂KBr浓度为1.2 mol/L、净化剂类石墨相氮化碳质量浓度为10.0 mg/mL、沸石咪唑酯骨架-67质量浓度为2.5 mg/mL时, AFB1的检出限为1 ng/mL, 线性范围为1~200 ng/mL, 相关系数(r2)为0.99377, 平均回收率为98.0%~104.6%, 相对标准偏差为0.73%~3.79%。结论 本研究建立的方法简单、快速且准确, 可用于花生中AFB1污染的快速检测。

英文摘要:

      Objective To establish a method for the determination of aflatoxin B1 (AFB1) in peanut based on QuEChERS purification and surface-enhanced Raman spectroscopy (SERS). Methods Taking nano silver (AgNPs) as SERS active substrate, combined with QuEChERS sample pretreatment technology and non labeled detection method based on SERS characteristic peak, AFB1 in peanut was determined, the effects of coagulant type and concentration, the ratio of AgNPs to sample, and the type and concentration of purifier on the experimental results were investigated. Results When the concentration of coagulant KBr was 1.2 mol/L, the mass concentration of carbon nitride in graphite phase of purifying agent was 10.0 mg/mL, and the mass concentration of zeolite imidazole ester skeleton-67 was 2.5 mg/mL, the limits of detection of AFB1 were 1 ng/mL, the linear range was 1-200 ng/mL, and the correlation coefficient (r2) was 0.99377, the average recoveries were 98.0%?104.6%, and the relative standard deviations were 0.73%?3.79%. Conclusion The method established in this study is simple, rapid and accurate, and can be used for rapid detection of AFB1 pollution in peanut.