缢蛏多糖提取工艺优化及生物活性研究

Study on extraction process optimization and bioactivity of Sinonovacula constricta polysaccharides

中文关键词:  缢蛏多糖  提取工艺优化  结构分析  抗氧化活性

英文关键词:Sinonovacula constricta polysaccharides  optimization of extraction process  structural analysis  antioxidant activity

中文摘要:

      目的 优化缢蛏多糖(Sinonovacula constricta polysaccharides, SCP)的提取工艺, 并对其生物活性进行研究。方法 采用响应曲面法对缢蛏多糖提取工艺进行优化, 红外光谱扫描和高效液相色谱法对缢蛏多糖进行光谱分析和单糖组成分析, 初步研究了缢蛏多糖的结构组成、体外抗氧化活性。结果 提取缢蛏多糖的最佳工艺条件是: 提取温度80 ℃、料液比1:2 (g/mL)、提取时间3 h, 此条件下多糖得率为0.976%。该多糖分子量约为480 kD, 单糖组成分析是由葡萄糖组成, 糖环构型为吡喃型。体外抗氧化试验结果表明, 缢蛏多糖的抗氧化活性较好, 其清除自由基能力与多糖浓度具有明显的量效关系。结论 本研究优化了缢蛏多糖的提取工艺, 初步分析了缢蛏多糖的结构组成和抗氧化活性, 实验结果可为缢蛏的精深加工及产品开发提供一定的理论依据。

英文摘要:

      Objective To optimize the extraction process of Sinonovacula constricta polysaccharides (SCP), and study its biological activity. Methods Response surface methodology was applied to optimize the extraction process of SCP, the spectral analysis and monosaccharide compositions of SCP were analyzed by infrared spectrum scanning and high performance liquid chromatography, and the structural compositions, antioxidant activities in vitro of SCP were studied. Results The optimum technological conditions for extracting polysaccharides from Sinonovacula constricta were as follows: Extraction temperature 80 ℃, solid-liquid ratio 1:2 (g/mL), extraction time 3 h, under these conditions, the yield of polysaccharide was 0.976%. The molecular weight of the polysaccharide was about 480 kD, the monosaccharide composition analysis was composed of glucose, and the sugar ring configuration was pyran type. The results of antioxidant test in vitro showed that the antioxidant activities of SCP were good, and its free radical scavenging abilities had obvious dose-response relationship with the concentration of polysaccharides. Conclusion The extraction process of Sinonovacula constricta polysaccharides is optimized, the structural compositions and antioxidant activities of Sinonovacula constricta polysaccharides are preliminarily analyzed, and this experimental results can provide a theoretical basis for the intensive processing and product development of Sinonovacula constricta.