铁皮石斛多糖对高脂饮食小鼠肠腔菌群的影响

Effects of Dendrobium officinale polysaccharides on lumen-associated microbiota in high fat diet-fed mice

中文关键词:  铁皮石斛多糖  高脂饮食  肠腔菌群  代谢

英文关键词:Dendrobium officinale polysaccharides  high fat diet  lumen-associated microbiota  metabolism

中文摘要:

      目的 探究铁皮石斛多糖(Dendrobium officinale polysaccharides, DOP)对高脂饮食小鼠肠腔菌群的影响, 为其功能性食品的开发提供参考。方法 以水提醇沉法提取DOP, 分别以高脂饮食(high fat diet, HFD)、高脂饮食联合DOP对昆明小鼠干预8周, 收集小鼠小肠段的内容物进行16S rRNA基因测序。结果 HFD使小鼠肠腔菌群中的乳杆菌属(Lactobacillus)丰度减少, 并使脱硫弧菌属(Desulfovibrio)、葡萄球菌属(Staphylococcus)及棒杆菌属(Corynebacterium_1)富集。此外, HFD使核苷酸代谢、脂质代谢等功能基因的丰度下调。DOP降低了有害菌属的丰度, 促进了uncultured_ f_Muribaculaceae、拟杆菌属(Bacteroides)及双歧杆菌属(Bifidobacterium)的增殖, 并提高了氨基酸代谢、脂质代谢等功能基因的丰度。结论 DOP可调控肠腔菌群结构及代谢功能, 并可通过短链脂肪酸等代谢产物保护或修复肠道黏膜, 具有潜在的益生元作用。

英文摘要:

      Objective To investigate the effects of Dendrobium officinale polysaccharides (DOP) on the lumen-associated microbiota in high fat diet-fed mice, to provide reference for the development of functional food. Methods DOP was extracted from Dendrobium officinale by a method of water extracting-alcohol precipitating. Kunming mice were treated with high fat diet (HFD), HFD combined with DOP for 8 weeks, respectively. The small intestine content of mice were collected for 16S rRNA gene sequencing. Results HFD reduced the relative abundance of Lactobacillus and increased the abundance of Desulfovibrio, Staphylococcus and Corynebacterium_1 in the intestinal microbiota of mice. Moreover, HFD down-regulated the abundance of functional genes such as nucleotide metabolism and lipid metabolism. DOP decreased the abundance of harmful bacteria, promoted the proliferation of uncultured_ f_Muribaculaceae, Bacteroides and Bifidobacterium, and increased the abundance of functional genes such as amino acid metabolism and lipid metabolism. Conclusion DOP can regulate the structure and metabolic function of intestinal flora, protect or repair intestinal mucosa through metabolites such as short chain fatty acids, and has potential prebiotic effects.