微波消解-离子色谱法测定枸杞多糖的含量及组成

Determination of the content and composition of Lycium barbarum polysaccharides by microwave digestion-ion chromatography

中文关键词:  微波辅助消解  枸杞多糖  离子色谱法

英文关键词:microwave-assisted digestion  Lycium barbarum polysaccharides  ion chromatography

中文摘要:

      目的 建立微波消解-离子色谱法测定枸杞多糖含量及组成的方法。方法 分别考察了三氟乙酸浓度、微波消解温度及微波消解时间对枸杞多糖消解效率的影响, 优化了离子色谱法检测枸杞多糖的色谱条件, 并对所建立的方法进行方法学评价。结果 枸杞多糖最佳消解条件为温度120 ℃、时间20 min、三氟乙酸浓度3 mol/L; 经方法学评价, 9种单糖和2种糖醛酸均具有良好的线性关系, 相关系数均大于0.98; 精密度相对标准偏差小于3.00%, 检出限和定量限良好。通过对枸杞中单糖组成的分析, 表明在枸杞中葡萄糖、木糖、半乳糖、甘露糖、阿拉伯糖、鼠李糖、果糖、岩藻糖、核糖、半乳糖醛酸、葡萄糖醛酸是组成枸杞多糖的主要成分。结论 微波辅助消解方法可以大大缩减枸杞多糖的消解时间, 可用于快速检测枸杞多糖的含量。

英文摘要:

      Objective To establish a method for the determination of the content and composition of Lycium barbarum polysaccharides by microwave digestion-ion chromatography. Methods A single factor experiment was used to investigate the effects of trifluoroacetic acid concentration, microwave digestion temperature and microwave digestion time on the hydrolysis efficiency of Lycium barbarum polysaccharides, respectively. The chromatographic conditions for the determination of Lycium barbarum polysaccharide by ion chromatography conditions were optimized, and the method was evaluated. Results The optimal hydrolysis conditions for Lycium barbarum polysaccharides were that digestion temperature was 120 ℃, digestion time was 20 min, and trifluoroacetic acid concentration was 3 mol/L. After methodological evaluation, 9 kinds of monosaccharides and 2 kinds of uronic acids had good linear relationship, the correlation coefficients were all greater than 0.98; the relative standard deviations of precision were less than 3.00%, and the limits of detection and quantification were good. The analysis of monosaccharides in Lycium barbarum showed that glucose, xylose, galactose, mannose, arabinose, rhamnose, fructose, fucose, ribose, galacturonic acid and glucuronic acid were the main components of Lycium barbarum polysaccharide.Conclusion The microwave-assisted digestion method can greatly reduce the digestion time of Lycium barbarum polysaccharide, and can be used to rapidly detect the content of Lycium barbarum polysaccharide.