体外模拟胃肠消化对缢蛏抗氧化活性的影响

Effects of simulated gastrointestinal digestion in vitro on the antioxidant activity of Sinonovacula constricta

中文关键词:  缢蛏  体外模拟消化  抗氧化活性  氨基酸含量  水解度

英文关键词:Sinonovacula constricta  simulated digestion in vitro  antioxidant activity  amino acid content  degree of hydrolysis

中文摘要:

      目的 基于体外模拟消化系统探究胃肠模拟消化对缢蛏的抗氧化活性的影响以及氨基酸含量的变化。方法 以新鲜缢蛏为原料, 向体外模拟消化系统加入胃蛋白酶和胰蛋白酶, 模拟在胃液和肠液下的胃消化、十二指肠消化和小肠消化过程, 研究缢蛏在消化过程中的羟基自由基(·OH)清除率、二苯代苦味酰基自由基(2,2-diphenyl-1-picrylhydrazyl radical, DPPH·)清除率、还原力、水解度、游离氨基态氮及游离氨基酸含量的变化。结果 整个胃肠消化阶段, 加酶组的·OH清除率、DPPH·清除率、还原力均高于对照组。肠消化产物的·OH清除率、DPPH·清除率、还原能力及水解度均高于胃消化产物, 并且肠消化产物中的总游离氨基酸含量及具有抗氧化特征的氨基酸含量均显著高于胃消化产物(P<0.05)。在十二指肠消化阶段, ·OH清除率、DPPH·清除率、还原力值达到最高。结论 体外模拟消化处理能够提高缢蛏的抗氧化活性, 其中十二指肠消化阶段产物抗氧化活性最高。

英文摘要:

      Objective To explore the effects of simulated gastrointestinal digestion on the antioxidant activity of Sinonovacula constricta and the change of amino acid content based on the simulated digestive system in vitro. Methods Fresh Sinonovacula constricta was used as material, adding pepsin and trypsin to the the simulated digestive system in vitro, digestive processes of stomach, duodenum and small intestine were simulated in gastric juice and intestinal juice, respectively, the changes of hydroxyl radical (·OH) clearance rate, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) clearance rate, reducing power, degree of hydrolysis, free amino nitrogen and free amino acid content of Sinonovacula constricta during digestion were studied. Results The whole gastrointestinal digestion phase,·OH clearance rate, DPPH· clearance rate and reducing power in the enzyme group were higher than the control group. The ·OH clearance rate, DPPH· clearance rate, reducing power and degree of hydrolysis were all higher than those of the gastric digestion products, moreover, the total free amino acid content and the amino acid content with antioxidant characteristics were significantly higher than that of the gastric digestion products (P<0.05). During the duodenal digestion phase, ·OH clearance rate, DPPH· clearance rate, and reducing power reached the highest. Conclusion Simulated digestion in vitro can improve the antioxidant activity of Sinonovacula constricta, the antioxidant activity of duodenal digestion products was the highest in duodenal digestion stage.