不同采摘时期贵定鸟王茶鲜叶次生代谢产物差异分析

Analysis on secondary metabolites difference of Guiding Niaowang tea fresh leaves in different picking period

中文关键词:  贵定鸟王茶  代谢组学  代谢差异  茶叶加工

英文关键词:Guiding Niaowang tea  metabolomics  metabolic difference  tea processing

中文摘要:

      目的 分析不同采摘时期贵定鸟王茶的次生代谢产物差异。方法 基于代谢组学技术对不同采摘时期贵定鸟王茶的次生代谢产物进行测定, 利用XCMS软件和METLIN数据库对代谢物进行定性分析, 用SIMCA-P软件进行定量分析和差异化合物筛选。结果 茶树样本间分离趋势明显, 且春茶和秋茶代谢物差异最大。不同采摘时期鸟王茶之间共筛选出12种关键差异化合物, 包括3种儿茶素、3种黄酮醇类、2种糖类、2种茶黄素类、1种氨基酸和1种黄酮糖苷。通路富集分析显示这些差异化合物主要分布于类黄酮生物合成代谢途径中。此外, 儿茶素、糖类和氨基酸类物质在春茶中含量最高, 黄酮醇、茶黄素和黄酮糖苷类物质在夏茶中含量最高, 秋茶中差异化合物的含量最低。结论 贵定鸟王春茶更适合加工成绿茶, 而夏茶更适合加工成红茶。

英文摘要:

      Objective To analysis the secondary metabolic difference of Guiding Niaowang tea in different picking period. Methods Metabonomics technology was used to analyze the secondary metabolites of Guiding Niaowang tea. XCMS software and METLIN database were used for qualitative analysis of metabolites. SIMCA-P software was used for quantitative analysis and differential compounds screening. Results The separation trend of tea tree samples was significant, and the difference of metabolites between spring tea and autumn tea was the largest. Twelve kinds of key differential compounds were detected among Niaowang tea in different picking period, including 3 kinds of catechins, 3 kinds of flavonols, 2 kinds of sugars, 2 kinds of theaflavins, 1 kind of amino acid and 1 kind of flavonoid glycoside. Pathway enrichment analysis showed that these differential compounds were mainly distributed in flavonoid biosynthesis pathway. In addition, catechins, sugars and amino acid were the highest in spring tea, flavonols, theaflavins and flavonoid glycoside were the highest in summer tea. The different compounds were the lowest in autumn tea. Conclusion Guiding Niaowang spring tea is more suitable for processing into green tea, while summer tea is more suitable for processing into black tea.